A hamster model of bile duct carcinoma in anomalous junction of the pancreaticobiliary duct

点击翻译按钮获取中文摘要

It has been well known that an anomalous junction of pancreaticobiliary ductal system arise both dilatation and cancerous change of the bile duct in the high incidence. Some mammals such as goats, rats, and hamsters have a remarkably long common pancreaticobiliary channel. However, it has never been determined bile duct dilatation and carcinoma do not occur in these animals. We reported previously that the activity of proteolytic enzyme in goats was significantly lower compared with that in humans, and that the bile duct dilatation could be induced in young goats by infusion of human pancreatic juice into the gallbladder of goats. In this study the composition of pancreatic juice was compared in human and hamsters. Hamsters received administration of N-nitrosobis amine (BOP) for 3 weeks. In addition, humanpancreatic juice was injected into the gallbladder of hamsters via cholecystostomy for 10 weeks to examine the role of pancreatic juice reflux in the bile duct carcinogenesis. Our biochemical analysis demonstrated significantly lower levels of proteolytic enzymes in hamster pancreatic juice compared with human juice.Injection of human pancreatic juice into gallbladders of hamsters with administration of BOP produced adenoma and carcinoma of the biliary tract in significantly higher incidence compared with those of injection of salinewith administration of BOP.Thus, bileduct carcinoma was apparently induced in hamsters by human pancreatic juice.Injection of only human pancreatic juice into gallbladdeis BOP did not produce bile duct carcinoma, but hyperplasia in the extrahepatic bile duct occurred in high in cidence.Injection of single or combined pancreatic enzymes instead of human pancreatic juice Showed no difference in the incidence of bile duct carcinoma but produced adenoma or Hyperplasia of extrahepatic bile duct in significatly higher incidence.