Basic study of discogenic low back pain.

选用Wistar大鼠45只。不同节段的交感神经干切除7天后，用乙酰胆碱酯酶组织化学方法对整个腰椎进行染色。L2~L6双侧交感神经干全切除后，腰椎间盘后部致密神经网络几乎完全消失。然而，双侧单节段切除或单侧多节段切除后，神经网络略有减少。将神经示踪剂霍乱毒素B亚单位放置于L5~L6关节突关节，对双侧背根神经节和椎旁交感神经节进行免疫组织化学检测。对新生大鼠腰椎连续切片和成年大鼠小关节囊、背根神经节和椎旁交感神经节切片进行了免疫组织化学研究。标记神经元存在于L1~L6的背根节和T12~L6的椎旁交感神经节。脊神经背支和交通支之间由降钙素基因相关肽免疫反应纤维和多巴胺-β-羟基酶免疫反应纤维相互连接。

Forty-five Wistar rats were used. Seven days after the resection of sympathetic trunks with ganglia at different levels, the whole lumbar spine was stained by acetylcholinesterase histochemical method. The dense nerve network on the posterior portion of lumbar intervertebral discs had disappeared almost completely after total resection of bilateral sympathetic trunks at L2-L6. However, there was slight decrease in the network after bilateral single-level resection or unilateral multisegmental resection. The results showed the posterior portion of lumbar intervertebral discs was innervated by the sympathetic nerves multisegmentally and bilaterally.To determine the sensory pathway, Cholera toxin B subunit, a neural tracer, was placed in the L5-L6 facet joint, and the bilateral dorsal root ganglia and paravertebral sympathetic ganglia were examined immunohistochemically. The serial sections of lumbar vertebrae of newborn rats and the sections of the facet joint capsules, dorsal root ganglia, and paravertebralsympathetic ganglia of adult rats were investigated immunohistochemically. Labeled neurons existed in ipstilateral dorsal root ganglia from L1 to L6 and in paravertebral sympathetic ganglia from T12 to L6. The dorsal ramus of the spinal nerve and rami communicantes were connected to each other by calcitonin gene-related peptide immunoreactive fibers and dopaminebeta-hydroxylase immunoreactive fibers.