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The uptake of lidocaine by Characean cells with nuclear magnetic resonance spectroscopy

核磁共振波谱法观察 Characean 细胞对利多卡因的摄取

基本信息

批准号：
07671655
负责人：
NOSAKA Shuichi
金额：
$ 1.41万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671655/
关键词：
Chara plasma membrane membrane potential Local anesthetic electrophysiology NMR calcium channel 活動電位脱分極 pH 低温カルシュウムイオン

项目摘要

The Chara internodal cell has an action potential. We have employed nuclear magnetic resonance (NMR) spectroscopy to investigate the uptake of lidocaine by the internodal cells of Chara corallina. One of these cells was incubated in 1mM lidocaine solution. After incubation, one sample was washed with deionized water and placed into the NMR tube. All the NMR measurements were performed on a JEOL GX-270 NMR spectrometer. Local anesthetic, lidocaine was taken up by the internodal cell. The uptake of lidocaine was dependent on extracellular pH.When the cells were incubated in lidocaine solution of pH7.8, the uptake of lidocaine increased significantly compared with those incubated in the solution of pH7.O, in the lidocaine solution of pH7.8 the lidocaine level for the intact cell was found to be much higher than that for the internal fluid squeezed from the cell. 0.3mM CaCI2 reduced the uptake of lidocaine, but it was not significant. 1OmM KCL did not increase the uptake of Ilidocaine. The … More se findings have an agreement with the fact that the concentration of uncharged lidocaine increases depending on extracellular pH.They suggested that lidocaine was distributed mainly in the cell membrane and/or the cell wall and the uncharged lidocaine was primarily important. The bound lidocaine might be related to the change of the conductance after the lidocaine-induced depolarization, When the cells were incubated in lower pH medium and at lower temperature, the uptake of lidocaine did not decrease.QX-314 depolarized the membrane of the cell at rest. This means that not only the charged form of local anesthetic but also the uncharged form can affect the membrane of the cell, In the Chara plasma membrane the main time course of the excitation followed the early ionic processes. The early ionic processes after reception of stimuli could be separated to 2-5 components, one of which was blocked by 0.5mM Ba^<2+>. We concluded that the K^+ channel opens very early after electrical stimulation. Less

脉轮节间细胞具有动作电位。我们用核磁共振波谱研究了珊瑚轮节间细胞对利多卡因的摄取。其中一个细胞在1 mM利多卡因溶液中孵育。孵育后，其中一个样品用去离子水洗涤并放入核磁共振管中。所有的核磁共振测量都是在JEOL GX-270型核磁共振波谱仪上进行的。局麻药利多卡因被结间细胞摄取。细胞对利多卡因的摄取依赖于细胞外pH。当细胞在pH为7.8的利多卡因溶液中孵育时，细胞对利多卡因的摄取显著高于在pH为7.0的利多卡因溶液中。0.3 mM CaCl2使利多卡因摄取减少，但差异不显著。1OMKCL不增加对伊利多卡因的摄取。The…更多的Se结果与不带电荷的利多卡因浓度随细胞外pH的增加而增加的事实相一致。这些结果表明，利多卡因主要分布在细胞膜和/或细胞壁，而不带电荷的利多卡因是主要的。结合型利多卡因可能与利多卡因去极化后电导的变化有关，在低pH和低温条件下，细胞对利多卡因的摄取没有减少。QX-314使静息状态下的细胞膜去极化。这意味着，不仅局麻药的带电形式，而且非带电形式都会影响细胞膜，在脉轮质膜上，兴奋的主要时间过程遵循早期的离子过程。接受刺激后的早期离子过程可分离为2-5个组分，其中一个组分可被0.5 mM的Ba2+和Gt；阻断。我们得出结论，在电刺激后，K~+通道开放得很早。较少