A STUDY ON ANTI-INFLAMMATORY ACTION OF A NEW ENDOGENOUS OPIOIDE-REGULATED SUBSTANCE

一种新的内源性阿片类管制物质的抗炎作用研究

• 批准号: 07671687
• 负责人: KUGIMIYA Toyoki
• 金额: $ 1.09万
• 依托单位: JUNTENDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671687/
• 关键词: Spinorphin; Carrageenan; Aminopeptidase N; Leuhistin; Anti-inflammatory; Aminopeptidese N; 好中球機能; 急性炎症機構

Spinorphin is an endogenous pain-regulated substance which modulated an enkephalin-degrading activity. In this study, we found that Spinorphin causes a significant inhibition of inflammatory responses by human PMNs stimulated with FMLP and may be a new endogenous anti-inflammatory peptide which is mediated in part through the suppression in [Ca^<2+>]i changes. Next we examined the effects of Spinorphin, using an in vivo system involving mouse air-pouch as a model of acute inflammatory response. In this model carrageenan and PMNs accumulation in inflammatory sites were used as a inflammation-tiggerring agent and PMNs accumulation at inflammatory sites was an indicator of an acute inflammatory response. Intravenous administration of Spinorphin within air-pouch site elicited by carrageenan, the inhibition being 64%, compared to the saline-treated group. Combination of Leuhistin, and inhibitor of aminopeptidase N,sinergestically suppressed PMNs accumulation induced carrageenan. In the future, Spinorphin will be an important tool for use in clarifying of neuropeptide-induced biological phenomena such as enkephalin mediated pain and inflammation.

Spinorphin是一种内源性的痛觉调节物质，具有脑啡肽降解活性。在这项研究中，我们发现Spinorphin可显著抑制FMLP刺激的人PMN的炎症反应，并且可能是一种新的内源性抗炎肽，其部分通过抑制[Ca^<2+>]i变化来介导。接下来，我们使用涉及小鼠气囊的体内系统作为急性炎症反应的模型来检查Spinorphin的作用。在该模型中，角叉菜胶和PMN在炎症部位的积聚被用作炎症触发剂，并且PMN在炎症部位的积聚是急性炎症反应的指标。在角叉菜胶引起的气囊部位内静脉施用Spinorphin，与盐水处理组相比，抑制率为64%。Leuhistin和氨肽酶N抑制剂的组合，可显著抑制角叉菜胶诱导的PMN聚集。在未来，Spinorphin将是一个重要的工具，用于澄清神经肽诱导的生物学现象，如脑啡肽介导的疼痛和炎症。

项目成果

Tadahiko Hazato: "a study on anti fnflammatory effect of an endoglnaus pain-legulated sulistasce-spinnpbin" Folia pharmacol.JPN. 108. 155-160 (1996)

Tadahiko Hazato：“一项关于内痛调节 sulistasce-spinnpbin 抗炎作用的研究”Folia drugl.JPN。

Tadahiro Kanazawa: "Spinorphin,a novel endognous regalator of enkephalin a model of actein otacte loamation" JPN.J.Pharm. 71. 247 (1996)

Tadahiro Kanazawa：“Spinorphin，一种新型内源性脑啡肽调节剂，肌动蛋白八肽沉积物模型”JPN.J.Pharm。

Tadahiro Kanazawa: "Spinorphin, a novel endogenous regulator of enkephalin, in a model of acute inflammation" JPN.J.Pharm. 71. 247 (1996)

Tadahiro Kanazawa：“Spinorphin，一种新型内源性脑啡肽调节剂，在急性炎症模型中”JPN.J.Pharm。

Tadahiko Hazato: "A study on anti-inflammatory effect of an endogenous pain-regulated substance-spinorphin" Folia Pharmacol.JPN. 108. 155-160 (1996)

Tadahiko Hazato：“内源性疼痛调节物质-spinorphin的抗炎作用研究”Folia Pharmacol.JPN。

Tadahiro Kanazawa: "action of spinorplin,a royel end pgenous sogulator of esky hadir icramodslofact cnflanmation" JPN,J.Pharm. 71. 247 (1996)

Tadahiro Kanazawa：“spinorplin 的作用，一种 royel end pogenous sogulator of esky hadir icramodslofact cnflanmation” JPN，J.Pharm。