The Influence of Amrinone on the Oxygen Affinity of Red Blood Cell

氨力农对红细胞氧亲和力的影响

• 批准号: 07671680
• 负责人: SHIMOKAWA Mitsuru
• 金额: $ 0.32万
• 依托单位: NARA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671680/
• 关键词: Amrinone; Cyclic AMP; P50; Oxygen Affinity

We investigated the influence of amrinone on the oxygen affinity of red blood cell. After centrifuge, red blood cell with Krebs-Ringer solution was divided into four groups. (Control Group, A1 : added to the amrinone of the concentration for the clinical use, Group A2 : ten times of the A1, Group A3 : one hundred times of the A1) After incubation in 37ﾟC for three hours, we measured P50,2,3-DPG,pH-e, pH-i, PCO2, base excess, blood sugar, Na-e, Na-i, K-e, K-i.P50 was not changed as 24.1,24.6,25.1,25.8 (Control, A3, A2, A1). But 2,3-DPG,pH-e, and base excess were decreased significantly in group A3 and A2 versus control group. Furthermore pH-i, PCO2 and blood sugar were decreased significantly in group A3 vs.control group.We concluded that amrinone did not decreased the oxygen affinity of red blood cell in spite of the acceleration of the metabolism.

我们研究了氨力农对红细胞氧亲和力的影响。离心后，将加入Krebs-Ringer溶液的红细胞分为四组。(对照组，A1：加入临床使用浓度的氨力农，A2组：A1的10倍，A3组：A1的100倍)在37ﾟC中孵育3h后，测定P50，2，3-DPG，pH-e，pH-i，PCO2，碱过量，血糖，Na-e，Na-i，K-e，K-i，P50未改变为241,246，25.1，25.8(对照，A3，A2，A1)。但A3组和A2组的2，3-DPG、pH-e和碱过剩均显著低于对照组。此外，A3组与对照组相比，pH-I、PCO2和血糖均显著降低，提示氨力农在加速代谢的同时，并未降低红细胞的氧亲和力。