The effect of nitric oxide on allograft rejection

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The mechanisms by which immune competent cells mediate allograft injury tissue is still controversial. Many experiments support the view the allosensitized cytotoxic T cells (CTL) and non-specific inflammatory mediators play a crititcal role in graft rejection.We demonstrated that DST prevented the development of CTL activity in the sponge matrix allograft as demonstrated by both direct in vitro cytotoxicity assays and limiting dilution analysis of graft infiltrating cells. DST was also associated with depressed NO synthesis by enhanced PGE2 production in the murine sponge allograft model.Tacrolimus suppressed the CTL activity and NO production. However, NO production of graft infiltrating cells increased after restimulation with IFNgamma/LPS.Allogeneic DTH was suppressed by NOS inhibitor.NO was related with immunoregulatory effects both in vitro and in vivo significantly. NO may be also effective as a marker of immune activation.