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Characteristics and expression of a mucin-like glycoprotein produced by ocular surface epithelium

眼表上皮产生的粘蛋白样糖蛋白的特征和表达

基本信息

批准号：
07671917
负责人：
WATANABE Hitoshi
金额：
$ 1.41万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671917/
关键词：
Corneal epithelium Conjunctival epithelium Mucin Tear fluid glycocalyx ムテン分化

项目摘要

Ocular surface epithelium produces a mucin-like glycoprotein recognized by R339 antibody. In normal, a mucin-like glycoprotein is found in the apical cell membrane adjacent to the tear film in the most apical cell, and is also found in the cytoplasm of the cells of the subapica cell layr. In keratinized corneal epithelium of vitamin A deficient rats, expression of the glycoprotein was absent or was remarkably reduced in the cell surface of the most apical cell. Electron microscopic study has shown that expression of the glycoprotein was not in the apical cell membrane in the most apical cell, but in the cytoplasm of the cells in the subapical 2 to 3 cell layrs. In contrast, a mucin-like glycoprotein was neither in the most apical cell, nor in the subapical cells in the central cornea showing high keratinization. With vitamin A treatment to vitamin A deficient rats, the corneal epithelium recovered expression of the glycoprotein correspondent to the the normalization of the cell morphol … More ogy. These observations suggest that expression and synthesis of a mucin-like glycoprotein was reduced in keratinized cells.In human study, a mucin-like glycoprotein by human ocular surface epithelium was also reduced in keratinized cells in superior limbic keratoconjunctivitis and ocular pemphigoid. The recovery of expression of the glycoprotein was found in the ocular surface epithelium.The induction of expression of the glycoprotein was investigated in the developing rats. The expression was not observed in the corneas of developing rats with the lids closed (at the age of less than 14 day), however, the expression was induced in the corneas of 50% of the rats with mucin secretagogue abdominally injection at the age of 12 day-old. The effect of the mucin secretagogue was reduced to 33% and 20% in the rats administrated at 11 day and 12 day old, respectively. Clinically, the expression of the glycoprotein was shown to be reduced in the ocular surface epithelium of dry eye patients. Therefore, these observation may lead to devolopment of the drug which induces expression of a mucin-like glycoprotein in the ocular surface epithelium. Less

眼表上皮产生被R339抗体识别的粘蛋白样糖蛋白。在正常情况下，粘蛋白样糖蛋白存在于最顶端细胞中邻近泪膜的顶端细胞膜中，并且也存在于顶端下细胞层的细胞的细胞质中。在维生素A缺乏大鼠的角化角膜上皮中，糖蛋白的表达不存在或在最顶端细胞的细胞表面显著减少。电镜观察表明，糖蛋白在大多数顶端细胞的顶端细胞膜上没有表达，而在亚顶端2 ~ 3层细胞的细胞质中有表达。与此相反，粘蛋白样糖蛋白既不在最顶端的细胞，也不在中央角膜的亚顶端细胞显示高角质化。维生素A缺乏大鼠经维生素A治疗后，角膜上皮细胞形态恢复正常，糖蛋白表达恢复 ...更多信息奥吉。这些观察结果表明，在角化细胞中，粘蛋白样糖蛋白的表达和合成减少。在人类研究中，人眼表面上皮细胞的粘蛋白样糖蛋白在上级边缘角膜结膜炎和眼类天疱疮的角化细胞中也减少。在发育期大鼠眼表上皮中发现糖蛋白表达的恢复。在眼睑闭合的发育中的大鼠（小于14日龄）的角膜中未观察到该表达，然而，在12日龄时腹腔注射粘蛋白促分泌素的大鼠中，50%的角膜中诱导表达。在11日龄和12日龄给药的大鼠中，粘蛋白促分泌素的作用分别降低至33%和20%。临床上，干眼患者的眼表上皮细胞中糖蛋白的表达减少。因此，这些观察结果可能导致开发诱导眼表上皮中粘蛋白样糖蛋白表达的药物。少