Evaluation of the anticancer activities of Na+/K+-ATPase inhibitor, sanguinarine in human oral squamous cell carcinoma cell lines

苯并菲啶生物碱（QBA），主要称为血根碱和白屈菜红碱，对癌细胞增殖的抑制作用已得到充分证实。在这里，我们证明了血根碱的抗肿瘤活性，在SAS，人口腔鳞状细胞癌（OSCC）细胞系的体外测定系统。MTT<50>法和侵袭实验分别用0.75- 1.0mM浓度范围内的IC值证实了其抗增殖和抗侵袭作用。血根碱还能够抑制细胞的非贴壁生长，但不影响细胞的粘附能力。最后，我们发现血根碱通过激活caspase和Bcl-2/Bax比率诱导凋亡性细胞死亡。这些结果表明，血根碱是一种潜在的钠泵（Na^+/K^+-ATP酶）抑制剂，对口腔鳞癌的治疗具有潜在的应用价值。

The effects of benzophenanthridine alkaloids (QBA), known mainly as sanguinarine and chelerythrine, on the inhibition of cancer cell proliferation have been well established. Here, we demonstrated the antitumor activity of sanguinarine using in vitro assay systems in SAS, a human oral squamous cell carcinoma (OSCC) cell line. The anti-proliferative and -invasive effects were confirmed with IC_<50> values in the concentration range of 0.75-1.0 mM by MTT assay and invasive assay, respectively. Sanguinarine was also able to suppress cell anchorage-independent growth, whereas it did not affect the cells' adhering capabilities. Finally, we found that sanguinarine induces apoptotic cell death by activating caspase and the Bcl-2/Bax ratio. Taken together, these results demonstrate that sanguinarine,sodium pump (Na^+/K^+-ATPase) inhibitor, is a potential inhibitor of tumorigenesis and suggest that it may be valuable in the development of new anticancer drugs for the treatment of oral squamous cell carcinoma.