Evaluation of the anticancer activities of Na+/K+-ATPase inhibitor, sanguinarine in human oral squamous cell carcinoma cell lines

苯并苯胺生物碱（QBA），主要是血碱和车车碱，对癌细胞增殖的抑制作用已经得到了很好的证实。在这里，我们在SAS（人口腔鳞状细胞癌（OSCC）细胞系）的体外检测系统中证明了血根碱的抗肿瘤活性。在0.75 ~ 1.0 mM浓度范围内，MTT法测定IC_<50>，侵袭性测定IC_<50>。血桂碱还能抑制细胞不依赖于锚定的生长，而不影响细胞的粘附能力。最后，我们发现血根碱通过激活caspase和Bcl-2/Bax比值诱导凋亡细胞死亡。综上所述，这些结果表明血根碱，钠泵（Na^+/K^+- atp酶）抑制剂，是一种潜在的肿瘤发生抑制剂，并提示它可能在开发新的抗癌药物治疗口腔鳞状细胞癌方面有价值。

The effects of benzophenanthridine alkaloids (QBA), known mainly as sanguinarine and chelerythrine, on the inhibition of cancer cell proliferation have been well established. Here, we demonstrated the antitumor activity of sanguinarine using in vitro assay systems in SAS, a human oral squamous cell carcinoma (OSCC) cell line. The anti-proliferative and -invasive effects were confirmed with IC_<50> values in the concentration range of 0.75-1.0 mM by MTT assay and invasive assay, respectively. Sanguinarine was also able to suppress cell anchorage-independent growth, whereas it did not affect the cells' adhering capabilities. Finally, we found that sanguinarine induces apoptotic cell death by activating caspase and the Bcl-2/Bax ratio. Taken together, these results demonstrate that sanguinarine,sodium pump (Na^+/K^+-ATPase) inhibitor, is a potential inhibitor of tumorigenesis and suggest that it may be valuable in the development of new anticancer drugs for the treatment of oral squamous cell carcinoma.