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HIGH-RESOLUTION IMMUNOCYTOCHEMISTRY OF DENTAL BASEMENT MEMBRANE

牙科基底膜的高分辨率免疫细胞化学

基本信息

批准号：
07671989
负责人：
SAWADA Takashi
金额：
$ 1.54万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671989/
关键词：
TOOTH GERM BASEMENT MEMBRANE IMMUNOCYTOCHEMISTRY LAMININ TYPE IV COLLAGEN ELECTRON MICROSCOPY FIBRONECTIN TOOTH DEVELOPMENT

项目摘要

1. A characteristic layr containing numerous fibrils is associated with the basement membrane (BM) of the inner epithelium during the early stages of odontogenesis. Tooth germs of monkeys were studied and each fibril in the layr (lamina fibroreticularis) was found to be a tubular structure, 8-9 nm in width, resembling a "basotubule", the tubular structure previously observed in various BMs. The space between the fibrils was filled with a network formed by irregular anastomosing strands with an average thickness of 4 nm ; these strands resembled the "cords" forming the network in the lamina densa of BMs.2. Immunoreaction for type IV collagen, laminin, and heparan sulfate proteoglycan were observed both in the lamina densa and lamina fibroreticularis. Fuzzy substances occurring very close to the lamina fibroreticularis manifested moderate immunoreactivity. The dental papilla cells located close to the BM demonstrated immunoreactive material mainly on the plasma membranes facing the BM.Re … More action products were also observed in large concavities formed in some area of the cell surfaces ; and small, immunopositive vesicles occurred close to the plasma membrane. Immunoreaction products could be found in the rough endoplasmic reticulum of some cells. These findings suggest that dental papilla cells may produce the BM components that are incorporated into the dental papilla BM - particularly into the lamina fibroreticularis - during tooth development.3. Labelling for fibronectin was mostly associated with the lamina fibroreticularis. Enamel proteins were also immunodetected in association with the lamina fibroreticularis and gradually accumulated as patches within mantle dentin and at its interface with ameloblasts. Patches of granular material, immunoreactive for fibronectin and/or enamel proteins were found along the odontoblastic processes and cell bodies. These data suggest that enamel proteins, by themselves or synergistically with other proteins, may play a part in the differentiation and/or formative events taking place at the ameloblast-odontoblast interface during the early stages of tooth development. Less

1.在牙体发育的早期，内上皮的基底膜(BM)上有一层特征性的、含有大量纤维的细胞层。本文对猕猴牙胚进行了研究，发现纤维网状层(Layr)中的每个原纤维均为管状结构，宽度为8~9 nm，类似于以往在各种BMS中观察到的管状结构。纤维之间的间隙充满了由平均厚度为4 nm的不规则吻合线条组成的网络，这些线条类似于在骨髓致密层中形成网络的索状物。在致密层和纤维网状层均可观察到IV型胶原、层粘连蛋白和硫酸肝素蛋白多糖的免疫反应。靠近纤维网状板的模糊物质呈中等免疫反应性。位于BM附近的牙乳头细胞免疫反应物质主要分布在面向BM的质膜上，Re…在细胞表面的一些区域形成的大的凹陷中也观察到更多的作用产物；靠近质膜的小的免疫阳性小泡出现。免疫反应产物可见于部分细胞的粗面内质网。这些发现表明，在牙齿发育过程中，牙乳头细胞可能会产生BM成分，这些成分被结合到牙乳头BM中--特别是纤维网状板中。纤维连接蛋白的标记主要与纤维网状板有关。与纤维网状层相关的釉质蛋白也被免疫检测到，并逐渐以斑块的形式聚集在牙本质外套膜内及其与成釉细胞的交界处。成牙本质细胞突起和细胞体中可见纤维连接蛋白和/或釉质蛋白免疫反应阳性的颗粒状物质。这些数据表明，釉质蛋白本身或与其他蛋白协同作用，可能在牙齿发育早期成釉细胞-成牙本质细胞界面发生的分化和/或形成事件中发挥作用。较少