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ImmunoーElectronmicroscopy of the Formation and Resorption of the Basement Membrane in the Tooth Enamel Organ

牙釉质器官基底膜形成和吸收的免疫电子显微镜

基本信息

批准号：
63570854
负责人：
SAWADA Takashi
金额：
$ 1.34万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

This study demonstrates ultrastructural localization of laminin, typeーIV collagen and acid phosphatase (ACPase) in the rat and monkey tooth enamel organs.Immunostaining for laminin and type-IV collagen was observed in the basement membranes of the inner enamel epithelium and differentiating ameloblasts. Reaction products occurred frequently in the rough endoplasmic reticulum of inner enamel epithelium cells. In differentiating ameloblasts, plasmaーmembrane undulation in the distal end caused deep, irregular invaginations. Various numbers of coated pits accompanied the invaginated plasma membrane. Reaction products occurred in both invaginations and coated pits. Reaction of ACPase was demonstrated in distalーcytoplasmic granules in differentiating ameloblasts. Sometimes the granules were very close to the invaginated plasma membranes. These results indicate that the basement membrane is produced by inner enamel epithelium cells and absorbed by differentiating ameloblasts.Intense laminin reaction occurred in both the internal and the external basal lamina. Laminin reaction products were observed within the rough endoplasmic reticulum of junctional epithelium cells and in minute invaginations formed at their distal plasma membranes adjacent to the basal lamina. Small spherical bodies occurring in the invaginations also reacted positively to laminin. No typeーIV collagen reaction products were seen in the internal basal lamina. The external basal lamina showed reaction to typeーIV collagen. These results indicate that the internal basal lamina contains laminin but no typeーIV collagen and that junctional epithelium cells synthesize laminin.

本研究观察了层粘连蛋白（laminin）、Ⅳ型胶原（type-IV collagen）和酸性磷酸酶（acidphosphatase，ACG）在内釉上皮和分化中的成釉细胞基底膜的超微结构定位。反应产物多见于内釉上皮细胞的粗面内质网。在成釉细胞的分化过程中，远端质膜的起伏引起深的、不规则的内陷。不同数量的被膜小坑伴随着内陷的质膜。反应产物发生在内陷和被覆坑。在成釉细胞分化过程中，ACEI反应主要出现在细胞质颗粒的远端。有时颗粒非常靠近内陷的质膜。这些结果表明，基底膜由内釉上皮细胞产生，并被分化中的成釉细胞吸收，内、外基底膜均发生强烈的层粘连蛋白反应。层粘连蛋白反应产物在交界上皮细胞的粗面内质网内观察到，并在其邻近基底层的远端质膜处形成微小的内陷。小球状体发生在内陷也积极反应层粘连蛋白。在内部基底层中未观察到Ⅳ型胶原反应产物。外基底膜对Ⅳ型胶原有反应。这些结果表明，内部基底层含有层粘连蛋白，但没有Ⅳ型胶原和交界上皮细胞合成层粘连蛋白。