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MECHANISM OF ADHESION BETWEEN GINGIVAL JUNCTIONAL EPITHELIUM AND TOOTH ENAMEL

牙龈交界上皮与牙釉质的粘附机制

基本信息

批准号：
13671912
负责人：
SAWADA Takashi
金额：
$ 1.86万
依托单位：
Tokyo Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

1) In this study the detailed ultrastructure of the dental cuticle at the dentogingival border in monkeys was observed. The results suggest that the dental cuticle, is formed at the dentogingival border by an accumulation of basement membrane components and a dark amorphous material of uncertain biochemical nature at the enamel side of the lamina densa of the basement membrane of the junctional epithelium. While the dark amorphous material may be derived, as has been suggested, from serum proteins in gingival exudates or hemoglobin from degraded red blood cells, further studies are still needed to demonstrate the origin of this dark material.2) In nonmammalian species, characterization of the tooth basement membranes is still to be advanced. In this study, detailed ultrastructural observations of the basement membranes of the tooth of the shark were made in its various developmental stages. The results indicate that basement membranes of the developing shark tooth, as those in the mammalian tooth, play various roles, including anchoring, firm binding, and possible mediation of the transport of substances that are known to be vital for the development of the tooth.3) In order to elucidate the mechanism of adhesion between the gingiva and the tooth, detailed ultrastructural studies of the dentogingival border were done in the monkey. At the border, the internal basement membrane is firmly bounded to the junctional epithelium through hemidesmosomes. The lamina densa was closely associated at its enamel side with an additional layer (supplementary lamina densa), which had characteristics of the lamina densa. In nondemineralized tissues a part of the basement membrane, that is, the supplementary lamina densa was preferentially mineralized. The mineral deposit was continuous with that in the enamel, thus constituting an advancing edge of mineralization. Therefore, strong gingiva-tooth adhesion is established by partial mineralization of the internal basement membrane.

1)本文对猴牙龈缘牙角质层的超微结构进行了详细观察。结果表明，牙角质层，形成在牙龈边缘的基底膜成分和一个黑暗的无定形物质的不确定的生物化学性质的积累在釉质侧的致密层的基底膜的结合上皮。虽然深色无定形物质可能来自牙龈渗出物中的血清蛋白或降解红细胞中的血红蛋白，但仍需要进一步研究来证明这种深色物质的来源。2）在非哺乳动物物种中，牙齿基底膜的表征仍有待进一步研究。本研究对鲨鱼牙齿基底膜在不同发育阶段进行了详细的超微结构观察。结果表明，发育中的鲨鱼牙齿的基底膜，在哺乳动物牙齿中，发挥各种作用，包括锚定，牢固的结合，并可能介导的物质的运输，已知是至关重要的牙齿的发展。3）为了阐明牙龈和牙齿之间的粘附机制，对猴的牙龈缘进行了详细的超微结构研究。在边界处，内部基底膜通过半桥粒与结合上皮牢固结合。致密层在其釉质侧与附加层（补充致密层）密切相关，该附加层具有致密层的特征。在非脱矿组织中，基底膜的一部分，即补充致密层优先矿化。矿物存款与釉质中的连续，从而构成矿化的前进边缘。因此，通过内部基底膜的部分矿化来建立强有力的牙龈-牙齿粘附。