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Long-term degradation of resin-dentin interface in vivo

体内树脂-牙本质界面的长期降解

基本信息

批准号：
07672071
负责人：
SANO Hidehiko
金额：
$ 1.34万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672071/
关键词：
Dentin bonding Durability Monkey teeth Adhesion

项目摘要

The long-term durability of bonds between adhesive resins and dentin is of significant importance for the longevity of bonded restorations. Objectives. This in vivo long-term study was carried out to evaluate the degradation of resin-dentin bonds in the oral cavity, as well as to test the hypothesis that the three-dimensional ashesive interfac would show morphological changes in vivo over time. Materials and Methods : Very shallow saucer-shaped dentin cavities were prepared in 12 intact teeth of one Japanese monkey (Macaca fuscata) under general anesthesia. The cavities were restored with Clearfil Liner Bond II and Clearfil Photo Posterior resin composite. The teeth were extracted at three different times ; immediate, 180, and 360 days after placement of the restorations. One day after sacrifice of the monkey, specimens of the three time periods were subjected to the micro-tensile bond test at a cross head speed of 1 mm/min. The surfaces of the failed bonds were observed under a field emission scamming electron microscope (FE-SEM). Results : Bond strength measurements in this study were successfully performed and were stable approximately 19 MPa during the one-year testing. Scanning electron microscopic observations of the failed surfaces revealed porosities at the top of the hybrid layr and within the adhesive resin which increased over time. Significance : Long-term bonds can be assessed in vivo using the microtensile bond test, and morphological changes of the adhesive interphase can be evaluated after the bond strength.

粘合树脂和牙本质之间粘合的长期耐久性对于粘合修复体的使用寿命非常重要。目标。这项体内长期研究的目的是评估口腔中树脂-牙本质键的降解，并检验三维粘合界面随着时间的推移在体内表现出形态变化的假设。材料和方法：在全身麻醉下，在一只日本猴（Macaca fuscata）的 12 颗完整牙齿中制备非常浅的碟形牙本质腔。使用 Clearfil Liner Bond II 和 Clearfil Photo Posterior 树脂复合材料修复蛀牙。牙齿在三个不同时间被拔除；放置修复体后立即、180 和 360 天。处死猴子一天后，将三个时间段的标本以1毫米/分钟的十字头速度进行微拉伸粘合测试。在场发射扫描电子显微镜（FE-SEM）下观察失效粘合的表面。结果：本研究中的粘合强度测量成功进行，并且在一年的测试期间稳定在约 19 MPa。对失效表面的扫描电子显微镜观察显示，混合层顶部和粘合树脂内的孔隙率随着时间的推移而增加。意义：可以使用微拉伸粘合测试在体内评估长期粘合，并且可以在粘合强度后评估粘合剂界面相的形态变化。