Changes of microbial flora from the subgingival pockets in the active phase of periodontitis.

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In this project, changes of microbial flora from the subgingival pockets in the active phase of periodontitis were investigated.To clarify the characteristics of microbial flora in the acute phase of periodontitis which seems to be one of the active phase of periodontitis, microbial flora of subgingival pockets of scute phase was compared with that of chronic phase. The proportions of Porphyromonas gingivalis and Bacteroides forsythus against total CFU from acute sites were significantly higher than those from chronic sites. Those bacteria seemed to play important role in the acute phase of periodontitis. And the monitoring of these bacteria seemed to be important for the diagnosis of active phase.For the monitoring of those periodontopathic bacteria, more simple and swifter bacterial tests are required. For detecting periodontopathic bacteria, non-radioactive DNA probe method (Affirm DP<@D1(]SY.encircled.R[)@>D1) was evaluated. DNA probe method showed superiority in sensitivity and specificity for the detection of P.gingivalis and B.forsythus compared with cultural method. DNA probe method could be apply for the microbial diagnosis for active phase.In view of the MIC of minocycline-HCl for periodontopatic bacteria, P.gingivalis and Actinobacillus actinomycetemcomitans did not showed the low susceptibility to minocycline-HCl. For the effect of local application minocycline gel, clinical improvement and significant decrease of P.gingivalis and B.forsythus was obsereved by one application. One application seemed to enough for alleviation of acute phase. As some cases showed the recolonization of periodontopathic bacteria in 4 weeks, debridement of periodontal pockets seemed to be effective after clinical improvement was observed by topical application of minocycline.