Membrane Potentials and Their Distribution Pattern of Inflammatory Cells Collected from Acute Periodontitis

急性牙周炎炎症细胞膜电位及其分布规律

• 批准号: 02670848
• 负责人: HORIUCHI Hiroshi
• 金额: $ 1.47万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670848/
• 关键词: Acute Periodontitis; Inflammatory Cell; Membrane Potential; Microelectrode; GCF; GCF; 微小電極; Spleen細胞; 腹腔内浸出細胞; MEM培地

Most of the studies on inflammatory cells of acute periodontitis have been related to morphological or immunological aspects of the problem. Although the membrane potential reflects cell function, the electrophysiological method has not attract attention among investigators. The purpose of this study is to establish a method to measure the membrane potential of inflammatory cells collected from acute periodontitis and to relate the recorded potential to cell functions.To calibrate the measuring system, the intracellular potential was measured using cells collected from peritoneal exudate and spleen. Inflammatory cells were collected from human gingival crdvicular fluid(GCF)using glass miniature capillaries. Then the cells were transplanted to the MEM broth with 10% FCS and incubated for 30 to 60 minutes in 5% carbon dioxide atmosphere. Glass micropipettes filled with 3 M KC1 solution were mounted on a 3 dimensional micromanipulator and the cells were impaled under the microscope.The measuring system was stable when the potential applied to the electrode was less than 50 mV. The membrane potential of cells from peritonealexudate ranged from -5.47 mV to -24.74 mV and the mean was -13.36 mV(s. d. =6.03 my). The potential of spleen cells ranged from -6.88 mV to -14.42 mV and the mean was -10.69 mV(s. d. = 8.53 my). The membrane potential of cells collected from GCF ranged from -2.2 mV to -17.6 mV and the mean was -6.4 mV.We are planning to elucidate the relationship between the membrane potentials of excited inflammatory cells and cell function.

大多数关于急性牙周炎炎症细胞的研究都与形态学或免疫学方面的问题有关。虽然膜电位反映了细胞的功能，但电生理方法一直没有引起研究者的重视。本研究的目的是建立一种测量急性牙周炎炎症细胞膜电位的方法，并将记录的电位与细胞功能联系起来。为了校准测量系统，使用从腹膜渗出液和脾脏收集的细胞测量细胞内电位。用玻璃微细毛细血管从人龈沟液（GCF）中采集炎症细胞。然后将细胞移植到含有10% FCS的MEM肉汤中，在5%的二氧化碳气氛中培养30 ~ 60分钟。将装有3 M KC1溶液的玻璃微移液管安装在三维显微操作仪上，在显微镜下刺穿细胞。当施加在电极上的电位小于50 mV时，测量系统是稳定的。腹膜酸盐细胞的膜电位范围为-5.47 mV ~ -24.74 mV，平均为-13.36 mV(s)。D. =6.03我的)。脾细胞电位范围为-6.88 mV ~ -14.42 mV，平均为-10.69 mV(s)。D. = 8.53我的)。GCF采集的细胞膜电位范围为-2.2 mV ~ -17.6 mV，平均值为-6.4 mV。我们计划阐明炎症细胞的膜电位与细胞功能之间的关系。

项目成果

佐伯 訓子,飯山 正夫,堀内 博: "歯根膜由来細胞の細胞膜電位" 日本歯科保存学会雑誌. 35. (1992)

Kuniko Saeki、Masao Iiyama、Hiroshi Horiuchi：“牙周膜来源细胞的细胞膜电位”日本保守牙科学会杂志 35。（1992 年）

佐伯 訓子,飯山 正夫,堀内 博: "歯根膜由来細胞の膜電位" 日本歯周病学会誌. 33. 111 (1991)

Kuniko Saeki、Masao Iiyama、Hiroshi Horiuchi：“牙周膜来源细胞的膜电位”日本牙周病学会杂志 33. 111 (1991)。