DETECTION OF CELLCYCLE REGURATRY GENES,ONCOGENES,TUMOR SUPPRESSORGENES IN HUMAN ORAL SQUAMOUS CELL CARCINOMA.

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A new tumor-suppressor gene, the cyclin dependent kinase 4 inhibitor gene, has been cloned and mapped on chromosome 9p where putative tumor suppressor gene of the oral squamous cell carcinoma (SCC) may be present. In order to elucidate whether abnormalities of this gene could contribute tot the tumor genesis of oral SCC,genomic DNAs from primary lesions and SCC cell lines were examined by PCR-SSCP analysis, direct DNA sequencing, and deletion analysis. Fifty seven percents cell lines contained non-sense mutations or mis-sense mutations. Particularly, 3 of 4 non-sense mutations detected in the present study were found in codon 80 (CGA*TGA ; Arg*Stop), suggesting that codon 80 was a mutational hot spot of the p16 gene. These results suggest that the p16 gene plays a role in the progression of a subtype of human oral SCC.