p16 gene alteration and proliferative potential of gliomas with fluorescence in situ hybridization and immunohistochemical studies.

通过荧光原位杂交和免疫组织化学研究研究神经胶质瘤的 p16 基因改变和增殖潜力。

• 批准号: 08670219
• 负责人: SHIBUYA Makoto
• 金额: $ 1.34万
• 依托单位: Tokai University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08670219/
• 关键词: glioma; p16 gene; proliferative potential; fluorescence in situ hybridization; FLSH; 神経膠腫; 増殖能

This study was performed to investigate correlations with proliferative potential and p16 gene alteration in human gliomas. And fluorescence in situ hybridization (FISH) technique is also applied for numeric abnormality in some chromosomes. Proliferative potential of gliomas determined by the monoclonal antibodies MIB-1 and MIB-5, anti-DNA topoisomerase II alpha and anti-cdc2 antibodies is well correlated with histological grade of malignancy in each cases. Whereas, p16 gene products-immunopositivity is not apparently correlated with their proliferative potential in each cases. Intracytoplasmic positive stainings of p16 gene products, as well as intranuclear reaction, are pronounced in cases with higher grade of malignancy. A distrubance of transportation of the p16 gene products may be suggestive form the results. With FISH study on dissociated tumor nuclei from the paraffin blocks, a gain of number of #7 chromosome and a loss of number of #10 chromosome using chromosome specific centromeric probes are observed in higher grade of malignancy.

本研究旨在探讨人脑胶质瘤中p16基因改变与肿瘤增殖潜能的关系。荧光原位杂交（FISH）技术也可用于某些染色体数目异常的检测.由单克隆抗体MIB-1和MIB-5、抗DNA拓扑异构酶II α和抗cdc 2抗体确定的胶质瘤的复发潜力与每种情况下的恶性肿瘤的组织学分级良好相关。而p16基因产物的阳性表达与其增殖潜能无明显相关性。在恶性程度较高的病例中，胞浆内p16基因产物阳性染色以及核内反应是明显的。结果提示p16基因产物转运障碍。用染色体特异性着丝粒探针对石蜡块上分离的肿瘤细胞核进行FISH研究，在恶性程度较高的肿瘤中观察到#7染色体数目增加和#10染色体数目丢失。

项目成果

渋谷 誠: "免疫組織化学的細胞増殖マーカーとそのpitfallについて" 病理と臨床. 15(1). 71-76 (1997)

Makoto Shibuya：“免疫组织化学细胞增殖标记及其陷阱”病理学和临床研究 15（1）（1997）。

渋谷 誠, 他1名: "細胞診標本を用いたfluorescencein situ hybridization(FISH)による細胞遺伝学的解析の試み" 日本外科学会雑誌. 97(5). 363-367 (1996)

Makoto Shibuya 等人：“使用细胞诊断标本进行荧光原位杂交 (FISH) 的细胞遗传学分析”，日本外科学会杂志 97(5) (1996)。

MAKOTO,SHIBUYA,et al.: "Immunohistochemical markers for proliferating cells." J.Pathol.Clin.Med.15 (Japanese). 71-76 (1997)

MAKOTO,SHIBUYA,et al.：“增殖细胞的免疫组织化学标记。”

渋谷誠: "免疫組織化学的細胞増殖マーカーとそのpitfallについて" 病理と臨床. 15(1). 71-76 (1997)

Makoto Shibuya：“免疫组织化学细胞增殖标记物及其陷阱”病理学和临床研究 15（1）（1997）。

MAKOTO,SHIBUYA,es al.: "Cytogenetic study with fluorescence in situ hybridization on cytologic specimen." J.Jap.Surg.97 (Japanese). 363-367 (1996)

MAKOTO,SHIBUYA,es al.：“对细胞学标本进行荧光原位杂交的细胞遗传学研究。”