Effect of Basic Fibroblast Growth Factor (b-FGF) on Restoration Process of Bone after Alveolar Osteotomy in Dogs

众所周知，碱性成纤维细胞生长因子(b-FGF)作为成纤维细胞和毛细血管内皮细胞的生长因子，加速了皮肤创面的愈合过程。最近有报道称，b-成纤维细胞生长因子通过局部应用刺激和增殖成骨细胞和成纤维细胞，从而促进骨折愈合。本研究的目的是从组织学和形态学的角度观察应用b-成纤维细胞生长因子的犬牙槽骨截骨后骨形成的恢复过程。对照组为2ET前磨牙区截骨术。B-成纤维细胞生长因子组：截骨后应用b-成纤维细胞生长因子。两组均于2 mm处舌侧移位。分别于术后第3、5、7、14、21、28天处死动物。光镜下观察骨折薄片，扫描电子显微镜观察细小血管腐蚀标本，结果如下：1.与对照组相比，b-FGF组骨折愈合良好，截骨后3天，b-FGF组与对照组相比，成纤维细胞数量增多，新生血管增多。提示b-成纤维细胞生长因子能刺激和增强成纤维细胞和成骨细胞的增殖和活性。B-成纤维细胞生长因子在修复的第一阶段非常有效。

It is well known that Basic Fibroblast Growth Factor (b-FGF) which is characterized as a growth factor for fibroblast and capillary endothelial cells, accelerates the process of dermal wound healing. Recently, it has been reported that b-FGF promotes bone fracture healing as a result of stimulating and proliferating the osteoblsts and Fibroblasts in local administration. The purpose of this study is to examine the restoration process of bone formation after alveolar osteotomy in the dogs applied with b-FGF histopasorogically and morphologically. The control group was osteotomy at 2et premolar region. The b-FGF group was applied with b-FGF after osteotomy. In both groups, the sectioned bone segment was lingually transferred at 2mm. The animals were sacrificed at 3,5,7,14,21 and 28 days after osteotomy. The thin sections were examined with a light microscope and fine vascular corrosion specimens were examined with a scanning electron microscope.The results were as follows :1. The bone fracture healing was exsellent in the B-FGF groups when compared to the control groups.3 days after osteotomy, a number of fibroblast and new blood vessels increased in the b-FGF group when compared to the control groups.3. It was suggested that the b-FGF stimulated and enhanced the cell proliferation and activity of fibroblasts and osteoblasts.4. The b-FGF was found to be very effective in the first stage of restoration process.