Effect of Basic Fibroblast Growth Factor (b-FGF) on Restoration Process of Bone after Alveolar Osteotomy in Dogs

碱性成纤维细胞生长因子（b-FGF）对犬牙槽骨截骨术后骨恢复过程的影响

• 批准号: 07672250
• 负责人: HIROSE Takehisa
• 金额: $ 1.47万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672250/
• 关键词: Alveolar Osteotomy; b-FGF; New blood vessel; Vascular network; scanning electron microscope; ビ-グル犬; alveolar osteotomy; 修復機転; 病理組織学的研究

It is well known that Basic Fibroblast Growth Factor (b-FGF) which is characterized as a growth factor for fibroblast and capillary endothelial cells, accelerates the process of dermal wound healing. Recently, it has been reported that b-FGF promotes bone fracture healing as a result of stimulating and proliferating the osteoblsts and Fibroblasts in local administration. The purpose of this study is to examine the restoration process of bone formation after alveolar osteotomy in the dogs applied with b-FGF histopasorogically and morphologically. The control group was osteotomy at 2et premolar region. The b-FGF group was applied with b-FGF after osteotomy. In both groups, the sectioned bone segment was lingually transferred at 2mm. The animals were sacrificed at 3,5,7,14,21 and 28 days after osteotomy. The thin sections were examined with a light microscope and fine vascular corrosion specimens were examined with a scanning electron microscope.The results were as follows :1. The bone fracture healing was exsellent in the B-FGF groups when compared to the control groups.3 days after osteotomy, a number of fibroblast and new blood vessels increased in the b-FGF group when compared to the control groups.3. It was suggested that the b-FGF stimulated and enhanced the cell proliferation and activity of fibroblasts and osteoblasts.4. The b-FGF was found to be very effective in the first stage of restoration process.

众所周知，碱性成纤维细胞生长因子（b-FGF）的特征在于其为成纤维细胞和毛细血管内皮细胞的生长因子，加速皮肤伤口愈合的过程。最近，据报道，b-FGF在局部给药中由于刺激和增殖成骨细胞和成纤维细胞而促进骨折愈合。本研究旨在从组织形态学角度观察b-FGF对犬牙槽骨截骨术后骨形成的修复过程。对照组为第2磨牙区截骨术。成纤维细胞生长因子组在截骨术后应用成纤维细胞生长因子。在两组中，将切下的骨段舌向转移2 mm。分别于术后3、5、7、14、21、28 d处死动物。采用光学显微镜观察薄切片，用扫描电镜观察细血管腐蚀标本。与对照组相比，B-FGF组骨折愈合明显加快，截骨后3 d，B-FGF组成纤维细胞和新生血管数量较对照组增加.提示b-FGF刺激和增强成纤维细胞和成骨细胞的增殖和活性.发现b-FGF在修复过程的第一阶段非常有效。