Urine Protein profile System for Pathophysiologic Diagnosis of Renal and Urogenital Disorders

用于肾脏和泌尿生殖系统疾病病理生理诊断的尿液蛋白谱系统

• 批准号: 07672496
• 负责人: KAWAI Tadashi
• 金额: $ 1.6万
• 依托单位: Jichi Medical School (JMS)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672496/
• 关键词: protein 1; alpha2-macroglobulin; hemoglobin; urine protein; basic fetoprotein; CR470; urine protein profile; protein 1; α2-マクログロブリン; RPPHS; ヘモグロビン; プロティン1; 尿蛋白; 塩基性蛋白; CRM470

The present project was successfully finished as planned before. During two years, the following research have been accomplished or are underway which are summarized as followed : a) Preparation of recombinant protein 1 and demonstration of its biological activity on inhibitory effects on phospholipase A2 and phospholipase C.Epitope analyzes are now under way. b) Immunohistochemical demonstration of protein 1 in epithelial cells of prostate and female prostate designated as Skene's gland. c) Demonstration of mechanisms of decreased serum protein 1 value in smokers due to decreased number of lung Clara cells and its decreased synthesis. d) Development of a latex agglutination photometric assay for urinary alpha2-macroglobulin. e) Evaluation of an Latex agglutination test for urinary basic fetoprotein (BFP) and discovery of the protein in semen structurally identical with native BFP.f) Establishment of BFP as a urinary marker for nonspecific inflammatory and tumor marker. g) Evaluation of a new international reference material for plasma proteins which gave the basis for future evaluation of a new reference material for urinary proteins. h) A study on urinary PSA-its evaluation of an assay and clinical application.Based on these research works, urine protein profiles systems are being constructed with completion of reference intervals for each key proteins, and its expert system. Clinical trials by the system will be initiated within a year at latest.

本项目以前已成功完成。在两年中，已完成或正在进行的研究中进行了总结，如下所示： b）在前列腺和雌性前列腺上皮细胞中蛋白1的免疫组织化学证明，称为Skene的腺体。 c）证明由于肺克拉拉细胞数量减少及其合成减少，吸烟者血清蛋白1值的机制降低。 d）开发用于尿α2-巨球蛋白的乳胶凝集光度测定。 e）评估尿液碱性胎儿蛋白（BFP）的乳胶凝集试验，并在结构上与天然BFP.f的精液中发现蛋白质，以建立BFP作为非特异性炎症和肿瘤标记的尿标志物。 g）评估血浆蛋白的新国际参考材料，该材料为将来评估尿蛋白的新参考材料提供了基础。 h）一项关于测定和临床应用评估的尿液PSA-ITS的研究。基于这些研究工作，正在为每个关键蛋白及其专家系统的参考间隔完成尿液蛋白剖面系统。该系统的临床试验最初将在一年内启动。

项目成果

山口哲司: "尿中α_2-マクログロブリンのラテックス免疫比濁測定法の確立" 臨床検査. 40. 855-858 (1996)

Tetsushi Yamaguchi：“尿液 α_2-巨球蛋白乳胶免疫比浊法的建立”临床实验室。 40. 855-858 (1996)

Zaviacic M., Danihel L., Ruzicoa M., Biazekova J., Itoh Y., Okutani R., KawaiT.: "Immunohistochemical localization of fuman protein 1 in female prostate (Skene's gland) and the male prostate." Histochim J.(in press). (1997)

Zaviacic M.、Danihel L.、Ruzicoa M.、Biazekova J.、Itoh Y.、Okutani R.、KawaiT.：“女性前列腺（斯基恩腺）和男性前列腺中 fuman 蛋白 1 的免疫组织化学定位。”

山口哲司: "尿中α2-マクログロブリンのラテックス免疫比濁測定法の確立" 臨床検査. 40. 855-858 (1996)

Tetsushi Yamaguchi：“尿液 α2-巨球蛋白乳胶免疫比浊法的建立”临床实验室。 40. 855-858 (1996)

Itoh Y., Ichihara K., Kanno T., Sugawara T., Ohkubo A., Hirabayashi Y., Igarashi S., Kawano K., Iwata S., Saito K., Kawai T.: "Serum Protein Standardization Project in Japan : Evaluation of an IFCC Reference Material (RPPHS/CRM470) and Establishment of Re

Itoh Y.、Ichihara K.、Kanno T.、Sukawara T.、Ohkubo A.、Hirabayashi Y.、Igarashi S.、Kawano K.、Iwata S.、Saito K.、Kawai T.：“血清蛋白标准化项目

R Okutami: "Preparation and claracterization of human recombinant protein 1" Eur J clin chem clin Biochem.34. 691-696 (1996)

R Okutami：“人重组蛋白 1 的制备和澄清”Eur J clin chem clin Biochem.34。