Urine Protein profile System for Pathophysiologic Diagnosis of Renal and Urogenital Disorders

用于肾脏和泌尿生殖系统疾病病理生理诊断的尿液蛋白谱系统

• 批准号: 07672496
• 负责人: KAWAI Tadashi
• 金额: $ 1.6万
• 依托单位: Jichi Medical School (JMS)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672496/
• 关键词: protein 1; alpha2-macroglobulin; hemoglobin; urine protein; basic fetoprotein; CR470; urine protein profile; protein 1; α2-マクログロブリン; RPPHS; ヘモグロビン; プロティン1; 尿蛋白; 塩基性蛋白; CRM470

The present project was successfully finished as planned before. During two years, the following research have been accomplished or are underway which are summarized as followed : a) Preparation of recombinant protein 1 and demonstration of its biological activity on inhibitory effects on phospholipase A2 and phospholipase C.Epitope analyzes are now under way. b) Immunohistochemical demonstration of protein 1 in epithelial cells of prostate and female prostate designated as Skene's gland. c) Demonstration of mechanisms of decreased serum protein 1 value in smokers due to decreased number of lung Clara cells and its decreased synthesis. d) Development of a latex agglutination photometric assay for urinary alpha2-macroglobulin. e) Evaluation of an Latex agglutination test for urinary basic fetoprotein (BFP) and discovery of the protein in semen structurally identical with native BFP.f) Establishment of BFP as a urinary marker for nonspecific inflammatory and tumor marker. g) Evaluation of a new international reference material for plasma proteins which gave the basis for future evaluation of a new reference material for urinary proteins. h) A study on urinary PSA-its evaluation of an assay and clinical application.Based on these research works, urine protein profiles systems are being constructed with completion of reference intervals for each key proteins, and its expert system. Clinical trials by the system will be initiated within a year at latest.

本项目已按计划顺利完成。在两年的时间里，已经完成或正在进行以下研究，总结如下：a）重组蛋白1的制备和其对磷脂酶A2和磷脂酶C的抑制作用的生物活性的证明。B）蛋白1在前列腺和称为Skene腺的雌性前列腺的上皮细胞中的免疫组织化学显示。c）证明吸烟者血清蛋白1值降低的机制是由于肺Clara细胞数量减少及其合成减少。d）尿α 2-巨球蛋白乳胶凝集光度测定法的开发。e）评价用于尿碱性胎蛋白（BFP）的胶乳凝集试验，并在精液中发现与天然BFP结构相同的蛋白。f）确立BFP作为非特异性炎症和肿瘤标志物的尿标志物。（g）评价新的国际血浆蛋白参考物质，为今后评价新的尿蛋白参考物质奠定基础。（h）尿PSA的研究--其检测方法的评价和临床应用。在这些研究工作的基础上，正在构建尿蛋白谱系统，完成每个关键蛋白的参考区间及其专家系统。该系统的临床试验最迟将在一年内启动。

项目成果

山口哲司: "尿中α_2-マクログロブリンのラテックス免疫比濁測定法の確立" 臨床検査. 40. 855-858 (1996)

Tetsushi Yamaguchi：“尿液 α_2-巨球蛋白乳胶免疫比浊法的建立”临床实验室。 40. 855-858 (1996)

Zaviacic M., Danihel L., Ruzicoa M., Biazekova J., Itoh Y., Okutani R., KawaiT.: "Immunohistochemical localization of fuman protein 1 in female prostate (Skene's gland) and the male prostate." Histochim J.(in press). (1997)

Zaviacic M.、Danihel L.、Ruzicoa M.、Biazekova J.、Itoh Y.、Okutani R.、KawaiT.：“女性前列腺（斯基恩腺）和男性前列腺中 fuman 蛋白 1 的免疫组织化学定位。”

Itoh Y., Ichihara K., Kanno T., Sugawara T., Ohkubo A., Hirabayashi Y., Igarashi S., Kawano K., Iwata S., Saito K., Kawai T.: "Serum Protein Standardization Project in Japan : Evaluation of an IFCC Reference Material (RPPHS/CRM470) and Establishment of Re

Itoh Y.、Ichihara K.、Kanno T.、Sukawara T.、Ohkubo A.、Hirabayashi Y.、Igarashi S.、Kawano K.、Iwata S.、Saito K.、Kawai T.：“血清蛋白标准化项目

山口哲司: "尿中α2-マクログロブリンのラテックス免疫比濁測定法の確立" 臨床検査. 40. 855-858 (1996)

Tetsushi Yamaguchi：“尿液 α2-巨球蛋白乳胶免疫比浊法的建立”临床实验室。 40. 855-858 (1996)

R Okutami: "Preparation and claracterization of human recombinant protein 1" Eur J clin chem clin Biochem.34. 691-696 (1996)

R Okutami：“人重组蛋白 1 的制备和澄清”Eur J clin chem clin Biochem.34。