Serum alpha_1-microglobulin variations in acute reaction phase

急性反应期血清α_1-微球蛋白变化

• 批准号: 05671929
• 负责人: KAWAI Tadashi
• 金额: $ 1.15万
• 依托单位: Jichi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671929/
• 关键词: alpha_1-microglobulin; Interleukin 6; IgA; Amyloid A; alpha-chain disease; alpha_1-マイクログロブリン; SAA; CRP; IL-6; 肝硬変症

alpha1-microglobulin (alpha1-m) is a low molecular weight glycoportein of 30kDa synthesized at liver as most of proteins are. Serum variations of this protein were investigated both in patients with surgical operation and alpha-chain disease.2) alpha1-microglobulin clinically proved to be a positive acute phase reactant. Serum value was ellvated postoperatively depending on the synthetic capacity of the liver reserved. Since its molecular weight was low, it is easily cleared from kidney, that have so far made it impossible to define this protein as a acute phase reactant. Futrher fundamental study is under way to elucidate precise mechanisms of alpha1-m reaction at the liver.For clinical significance, total measurement of this protein can be useful as a sensitive indicator of liver function and its recovery.2) Structure analysis of an alpha-chain-alpha1-m complex in alpha-chain diseaseBasic structure of alpha-chain-alpha1-m complex is a heterodimer of Fcalpha and Fcalpha-alpha1-m complex, in latter of which alpha1-m covalently binds at 1 : 1 molar ratio. alpha1-m was extremely low in serum concentration as compared with that in alpha-chain.Immunohistochemical examinations have shown no alpha1-m positive localization in involved tissues, but structurally abnormal alpha-chain. A complex form should be synthesized elsewhere in the tissues in the body once alpha-chain is released into circulation from involved lymph nodes.3) Development of a serum amyloid A (SAA) assayIn the process of the present study, a precise assay for SAA was developed using a latex agglutination reaction.4) Mechanisms of alpha1-m synthesisEffects of Interleukin 6 was investigated on synthetic capacity of alpha1-m by a hepatoma cell line (cCH4) to show that the alpha1-m was reduced in its value in the supernatant fluids, which contradicted the clinical findings.

α 1-微球蛋白（alpha 1-microglobulin，α 1-m）是一种低分子量的糖蛋白，分子量约为30 kDa，与大多数蛋白质一样，由肝脏合成。2）临床证实α_1-微球蛋白是一种阳性的急性时相反应物。术后根据保留肝脏的合成能力而升高血清学值。由于其分子量低，很容易从肾脏中清除，因此迄今为止无法将该蛋白定义为急性期反应物。2）α链病中α-链-α 1-m复合物的结构分析α-链-α 1-m复合物的基本结构是Fcalpha和Fcalpha-α 1-m复合物的异二聚体，在后者中，alpha 1-m以1：1摩尔比共价结合。α 1-m在血清中的浓度极低，免疫组织化学检查显示在受累组织中无α 1-m阳性定位，但α链结构异常。一旦α-链从相关淋巴结释放到循环中，复合物形式应该在体内组织的其他地方合成。3）血清淀粉样蛋白A（SAA）测定的开发在本研究的过程中，用乳胶凝集反应建立了一种精确的SAA测定方法。4）α 1-m合成的机制研究了白细胞介素6对α 1-m合成能力的影响。m的肝癌细胞系（cCH 4），以表明α 1-m的值在上清液中降低，这与临床发现相矛盾。

项目成果

佐々木勝一他: "ラテックス凝集反応法を用いた血清アミロイドAの基礎的検討"

Katsuichi Sasaki 等人：“使用乳胶凝集法进行血清淀粉样蛋白 A 的基础研究”

K.Sasaki, Y.Itoh, T.Kawai.: "A latex agglutination assay for serum amyloid A" IGAKU TO YAKUGAKU. (in press). (1995)

K.Sasaki、Y.Itoh、T.Kawai.：“血清淀粉样蛋白 A 的乳胶凝集测定” IGAKU TO YAKUGAKU。

佐々木勝一: "ラテックス凝集反応法を用いた血清アミロイドAの基礎的検討" 薬学と医学. (in press). (1995)

Katsuichi Sasaki：“使用乳胶凝集法进行血清淀粉样蛋白 A 的基础研究”药理学和医学（1995 年出版）。