In vivo antioxidant activity of flavonoids and their evaluation as food factor

黄酮类化合物的体内抗氧化活性及其作为食品因子的评价

• 批准号: 07680029
• 负责人: SHIMOI Kayoko
• 金额: $ 0.96万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680029/
• 关键词: flavonoid; luteolin; free radical; Fe-NTA; antioxidant effec; plasma level; daily intake; content

In order to evaluate chemopreventive activity of flavonoids, the absorption of flavonoids into the circulatory systems of humans and mice, and the protective effects on oxidative stress, were examined in male mice exposed to gamma-ray, ferric nitrilotriacetate (Fe-NTA) and doxorubicin, which generate active oxygen species and induce lipid peroxidation.HPLC analysis, using Capcell Pak C18-UG 120 column, was carried out to determine the concentrations of eight flavonoids (rutin, myricitrin, quercetin, myricetin, fisetin, kaempferol, luteolin, eriodictyol) in food samples and blood plasma. Lyophilized food samples from seven kinds of meals contained from 5 mg to 30 mg of flavonoids. The concentration of quercetin in human plasma reached the highest level (less than 100ng/ml) 30-60min after ingestion of the meal, which contained 60.8mg of quercetin and 11.4mg of kaempferol. The concentration of quercetin retured to the pro-meal level within 5h. The mouse plasma concentration of alphaG-Rutin reached the highest level 30 min after oral administration and then returned to the control level within 60 min.When mice received alphaG-Rutin or luteolin by single gastric intubation 30min before gamma irradiation (1.5Gy), a significant reduction in the frequency of micronucleated reticulocytes (MNRETs) was observed. Administration of alphaG-Rutin or luteolin by gastric intubation suppressed lipid peroxidation in the bone marrow, spleen, kidney and heart of gamma-irradiated, Fe-NTA or doxorubicin treated mice. Further, alphaG-Rutin showed a protective effect against subacute nephrotoxicity as revealed by the induction of karyomegalic cells in the renal proximal tubules of mice treated with Fe-NTA.These results suggest that absorbed flavonoids works as an antioxidant in vivo. Daily intake of flavonoids may contribute to the chemoprevention of free radical-associated diseases such as cancer.

为了评价黄酮类化合物的化学预防活性，在暴露于γ射线、次氮基三乙酸铁（Fe-NTA）和多柔比星（Doxorubicin）的雄性小鼠中检测黄酮类化合物进入人和小鼠循环系统的吸收，以及对氧化应激的保护作用。测定了8种黄酮类化合物（芦丁、杨梅苷、槲皮素、杨梅苷、非瑟酮、山奈酚、木犀草素、圣草酚）在食品和血浆中的含量。来自七种膳食的冻干食物样品含有5毫克至30毫克的黄酮类化合物。人血浆中槲皮素浓度在进食后30- 60分钟达到最高值（<100 ng/ml），其中槲皮素含量为60.8mg，山奈酚含量为11.4mg。槲皮素浓度在5 h内恢复到餐前水平。小鼠口服α G-R β 30 min后血药浓度达到最高水平，60 min后恢复到对照水平。在γ射线照射（1.5Gy）前30 min单次灌胃给予α G-R β或木犀草素，可显著降低微核网织红细胞（MNRET）的发生率。通过胃插管给药α G-R β或木犀草素抑制了γ-辐射、Fe-NTA或阿霉素处理的小鼠的骨髓、脾、肾和心脏中的脂质过氧化。此外，alphaG-Rynx显示出对亚急性肾毒性的保护作用，如通过用Fe-NTA处理的小鼠的肾近端小管中的巨核细胞的诱导所揭示的。这些结果表明，吸收的类黄酮在体内起抗氧化剂的作用。每日摄入类黄酮可能有助于化学预防自由基相关疾病，如癌症。

项目成果

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Shimoi, K.等人：“预防癌症的食物因素”Springer-Verlag，东京，617-622(6) (1997)

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Sadzuka, Y., Sugiyama, T., Shimoi, K., Kinae, N.and Hirota, S.: "Protective effect of flavonoids on doxorubicin-induced cardiotoxicity." Toxicol.Lett.92. 1-7 (1997)

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K. Shimoi et al.,: "Radioprotective effect of antioxidative flavonoids in mice." Mutat. Res.350. 153-161 (1996)

K. Shimoi 等人：“抗氧化类黄酮对小鼠的辐射保护作用。”