STUDIES ON FOOD ADDITIVES (ERYTHORBIC ACID) ABSORPTION IN MAN

人类吸收食品添加剂（异抗坏血酸）的研究

• 批准号: 07680057
• 负责人: FUJII Toshiko
• 金额: $ 0.9万
• 依托单位: KAWASAKI UNIVERSITY OF MEDICAL WELFARE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680057/
• 关键词: FOOD ADDITIVES; ERYTHORBIC ACID; ASCIRBIC ACID; URINARY EXCRETION; SIMULTANEOUS DETERMINATION; HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS; BIOLOGICAL MONITORING; キャピラリー電気泳動法; 生体内動態; ヒト尿; フリーゾーンキャピラリー電気泳動法

Erythorbic acid (ErA) is one of the stereoisomers of L-ascorbic acid (AsA). It possesses antioxidative properties similar to AsA and is used widely in Japan as an antioxidant in foods with no dosage limitation, because animal tests show that it has no toxic effects. The results of our biological monitoring studies in humans showed that absorption and/or excretion of ErA may be less than that of AsA after administration of simultaneous oral doses. However, further tests must be done to confirm these findings. Because the concentration of food additives in biological samples is very low, we first tried to simultaneously determine these two acids by high performance capillary electrophoresis (HPCE).Methods : The HPCE procedure called micellar electro kinetic chromatography (MEKC) using a buffer solution containing micelles of ionic surfactant (e.g. SDS), has been used to simultaneously determine AsA and ErA in standard solutions and urine samples spiked with AsA and ErA.10nl of sample was siphoned into the capillary. The proposed method used a 500 mm*75mum i.d. fused silica column and a 10mM borate buffer containing 50 mM SDS (pH 11.0). The determination was carried out using a 15kV separation voltage at 25ﾟC.The UV absorption was measured at lambda=265nm.Results : The AsA peak migrated at 5.7min and the ErA peak at 6.1 min in the standard solution. In the urine samples, the AsA peak migrated at 5.2 min and the ErA peak at 5.6min. The separation was complete in eight minutes and there was no overlapping with the creatinine peak (4.9min) and uric acid peak (5.8min). The recovery of AsA and ErA from the urine was 98% and 88%.respectively.

抗坏血酸(Era)是L抗坏血酸(AsA)的立体异构体之一。它具有与ASA类似的抗氧化性能，在日本被广泛用作食品中的抗氧化剂，没有剂量限制，因为动物试验表明它没有毒性影响。我们在人体内的生物监测研究结果表明，同时口服给药后，Era的吸收和/或排泄可能比ASA少。然而，必须进行进一步的测试才能证实这些发现。由于生物样品中食品添加剂的浓度很低，我们首次尝试用高效毛细管电泳法(HPCE)同时测定这两种酸。方法：采用胶束电动毛细管电泳法(MEKC)，在含有离子表面活性剂(如：十二烷基硫酸钠)胶束的缓冲溶液中同时测定标准溶液和添加ASA和ErA的尿样中的ASA和Era。所提出的方法使用了500 mm*75um的内径。熔融硅胶柱和含有50 mm十二烷基硫酸钠(pH 11.0)的10 mm硼酸盐缓冲液。在25ﾟC，分离电压为15KV，在波长265 nm处测定紫外吸收。结果：在标准溶液中，AsA峰移动5.7min，ERA峰移动6.1min。在尿样中，ASA峰迁移至5.2min，ERA峰迁移至5.6min。分离时间为8min，与肌酸峰(4.9min)和尿酸峰(5.8min)无重叠现象。尿中阿司匹林和埃拉的回收率分别为98%和88%。