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Analysis of the phenolic antioxidants, some food additives, and chemicals found in plastic tableware which are suspected of being endocrine disruptors.

对塑料餐具中发现的疑似内分泌干扰物的酚类抗氧化剂、某些食品添加剂和化学物质进行分析。

基本信息

批准号：
11680145
负责人：
FUJII Toshiko
金额：
$ 1.66万
依托单位：
Kawasaki University of Medical Welfare
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Titles of two major papers and their abstracts are as follows.(1) Title : Simultaneous Determination of Sodium Benzoate and p-Hydroxybenzoate Esters Using High-performance Capillary Electrophoresis. Abstract : A rapid and simple capillary zone electrophoresis (CZE) method was developed for the simultaneous determination of sodium benzoate, potassium sorbate, sodium dehydroacetate, and the parabens, i. e. ethyl, n-propyl, and n-butyl p-hydroxybenzoic esters, which are used in several processed foods as preservatives. The compounds were well separated from each other on a fused silica capillary utilizing a 20 mM sodium tetraborate buffer and UV detection at 200 nm. The total analysis time was less than seven minutes per sample. Concentrations of sodium benzoate and n-butyl paraben in several soft drinks were determined using the CZE method and the levels of sodium benzoate and n-butyl paraben in the samples were in good agreement with those determined by the HPLC procedure.(2) Title : Co … More mparison Among High-performance Liquid Chromatography, High-performance Capillary Electrophoresis and Gas Chromatography for the Simultaneous Determination of Phenolic Antioxidante. Abstract : Two major phenolic antioxidant food additives, 2-and 3-tert-4-hydroxyanisole (BHA) and 3, 5-di-tert-butyl-hydtroxytoluene (BHT), were determined in standard solutions using HPLC with an electrochemical detector, micellar electrokinetic capillary electrophoresis (MEKC) and capillary gas chromatography (GC). Analysis time was 22 minutes by HPLC-ECD and ten minutes by both MEKC and GC. Calibration curves obtained by comparing peak areas against concentrations showed a high degree of linearity (R^2>0.99) in all three methods. Of the three methods, BHA and BHT were detectable in the smallest amounts (10^<-14>moles) by MEKC. Variation in retention or migration time was ≦1.0% (R. S. D., %) on any given day, but was slightly higher than 1.0% by MEKC on separate days. Repeatability in peak-area also varied by ≦5.0% for both HPLC-ECD and GC, but varied by ≧5.0% for MEKC. Intermediate precision in peak-height of the antioxidants varied by ≦5.0% for MEKC. These methods were found to be suitable for the simultaneous determination of BHA and BHT concentrations in foods. Less

以下是两篇主要论文的题目和摘要。(1)题目：高效毛细管电泳法同时测定苯甲酸钠和对羟基苯甲酸酯。摘要：建立了一种快速简便的毛细管区带电泳（CZE）同时测定苯甲酸钠、山梨酸钾、脱氢乙酸钠和对羟基苯甲酸酯（乙基、正丙基和正丁基对羟基苯甲酸酯）的方法，这些对羟基苯甲酸酯在多种加工食品中用作防腐剂。利用20 mM的四硼酸钠缓冲液和200 nm的紫外检测，在熔融石英毛细管上很好地分离了化合物。每个样品的总分析时间少于7分钟。用CZE法测定了几种软饮料中苯甲酸钠和对羟基苯甲酸正丁酯的含量，样品中苯甲酸钠和对羟基苯甲酸正丁酯的含量与HPLC法测定的含量一致。(2)题目：高效液相色谱法、高效毛细管电泳和气相色谱法同时测定酚类抗氧化剂的比较。摘要：采用电化学检测器、胶束电动毛细管电泳（MEKC）和毛细管气相色谱（GC）在标准溶液中测定了2-和3-叔-4-羟基甲苯（BHA）和3,5 -二叔丁基-羟基甲苯（BHT）两种主要的酚类抗氧化食品添加剂。HPLC-ECD分析时间为22分钟，MEKC和GC分析时间均为10分钟。通过比较峰面积与浓度获得的校准曲线在所有三种方法中都显示出高度的线性（R^2>0.99）。在三种方法中，MEKC检测到的BHA和BHT的量最小（10^<-14>摩尔）。保留或迁移时间的变化在任何一天都是≦1.0% (r.s.d, %)，但MEKC在个别日子略高于1.0%。HPLC-ECD和GC的峰面积重复性变化≤5.0%，而MEKC的峰面积重复性变化≤5.0%。MEKC抗氧化剂峰高的中间精度变化≦5.0%。这些方法适用于同时测定食品中BHA和BHT的浓度。少