Immunohistochemical studies on the localization of Interleukin-1 and its receptor in the hippocampal CA-1 region of the ischemic brain.

通过蒙古沙鼠双侧颈动脉短暂闭塞，免疫组织化学检测了白细胞介素-1 （il -1 α、il -1 β）和受体（IL-1RtI、IL-1RtII）在海马CA-1区的定位。两种IL-1 （α或β）的免疫反应在放射层反应性星形胶质细胞、小胶质细胞和毛细血管内皮细胞中发现，在再循环后3天至30天。免疫阳性反应性星形胶质细胞伴水肿改变的数量增加，直至循环后30天。电镜下，星形胶质细胞、小胶质细胞和内皮细胞的胞浆中弥漫性分布有免疫反应性内源性产物。两种IL-1R （I，II）在反应性星形胶质细胞、小胶质细胞和毛细血管内皮细胞中的免疫反应性在循环后30天增加，锥体神经元的数量在循环后3 - 7天增加。与内皮细胞的弥漫性定位相反，星形胶质细胞的IL-1R免疫反应产物分散在细胞质中，特别是靠近细胞质膜和毛细血管周围的根尖突。免疫阳性锥体神经元表现中度至重度形态改变。这些发现提示IL-1系统在脑缺血损伤中起自分泌/旁分泌作用。

The localization of Interleukin-1 (IL-1alpha, IL-1beta) and receptors (IL-1RtI,IL-1RtII) was immunohistochemically examined in the hippocampal CA-1 region by a transient occlusion of bilateral carotid arteries in Mongolian gerbils. Immunoreactivities for both types of IL-1 (alpha or beta) were found in reactive astrocytes, microglias and endothelial cells of capillaries in the stratum radiatum from 3 days to 30 days after the recirculation. Immunopositive reactive astrocytes with edematous changes increased in number until 30 days after the recirculation. At the electron microscopic level immunoreactive endoproducts were diffusely distributed in the cytopolasm of astrocytes, microglias and endothelial cells. Immunoreactivities for both types of IL-1R (I,II) were observed in reactive astrocytes, microglias and endothelial cells of capillaries increase in number until 30 days and also pyramidal neurons from 3 to 7 days after recirculation. Contrary to the diffuse localization of endothelial cells, immunoreactive products of IL-1R of reactive astrocytes were dispersed in the cytoplasm, especially close to the cytoplasmic membrane and apical processes around capillaries. Immunopositive pyramidal neurons showed moderate to severe morphological alterations. These findings suggest that the IL-1 system plays autocrine/paracrine role in brain ischemic insult.