Mechanism of the demyelination and regulation by the microglia in neuropathic pain

我们研究了脊髓后角溶血磷脂酸受体-1（LPA-1）的变化，LPA-1是一种参与神经病理性疼痛时疼痛和脱髓鞘的物质，LPA-1 mRNA在术后6小时内开始表达。LPA-1免疫反应性观察到的少突胶质细胞，星形胶质细胞的脊髓后角的细胞质中，也，神经节细胞的背根神经节，许旺细胞，和髓鞘的质膜。伤侧LPA-1免疫阳性细胞数在伤后3、7天增加。这些LPA-1免疫阳性细胞可能与神经病理性疼痛的发生有关，小胶质细胞被认为是通过LPA在损伤侧产生的ATP释放而激活、扩增和诱导慢性疼痛。

We investigated the change of the lysophosphatidic acid receptor-1 (LPA-1) in the posterior horn of spinal cord known as a substance which participates in the pain and demyelination at the time of a neuropathic pain.The onset of LPA-1 mRNA was within postoperative 6 hour. LPA-1 immunoreactivity was observed in the cytoplasm of the oligodendrocyte, astrocyte of a posterior horn of spinal cord, and also, the protoplasmic membrane of the ganglion cell of a dorsal root ganglion, Schwann-cell, and myelin sheath. In injured side, the number of LPA-1 immune-positive cells increased at 3, 7days after injury. These LPA-1 immunopositive cells may be relevant to the onset of neuropathic pain.Microglia were considered to be induces in activation, amplification and chronic pain through the ATP release by produced LPA at injured side.