Protective effect with adenoviral vector-mediated GDNF gene on the hippocampal delayed neuronal death in gerbils

应用腺病毒载体介导的人胶质细胞源性神经营养因子（GDNF）基因在沙土鼠双侧颈总动脉短暂闭塞前2天的侧脑室表达GDNF。在再灌注2、4和7天，GDNF治疗组海马CA-1锥体细胞末端脱氧核苷酸转移酶介导的dUTP-生物素原位缺口末端标记（TUNEL）染色明显减少。在GDNF治疗组中，再灌注后7天海马中的锥体细胞保存良好[载体和GDNF分别为7.1%和62.8%]。这一结果表明，GDNF治疗对海马CA-1的迟发性神经元死亡具有显著的保护作用。

A recombinant adenoviral vector encoding the human glial cell line-derived neurotrophic factor (GDNF) gene was used to express the neurotrophic factor GDNF in the lateral ventricle of gerbil brain before 2 days of transient bilateral common carotid artery occlusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) staining in pyramidal cells of hippocampal CA-1 was markedly reduced in the case with GDNF treatment at 2, 4 and 7 days of reperfusion. In GDNF treated group, pyramidal cells were well preserved in the hippocampus at 7 days after reperfusion [7.1% vs. 62.8% for vehicle and GDNF, respectively]. This results suggest that the treatment with GDNF has a significant protective effect on delayed neuronal death of hippocampal CA-1.