A study on the perception mechanisms of the retina with a computational and a physiological approaches.

通过计算和生理学方法研究视网膜的感知机制。

• 批准号: 07680876
• 负责人: YAGI Tetsuya
• 金额: $ 1.41万
• 依托单位: Kyushu Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680876/
• 关键词: early vision; retina; standard regularization problem; Laplacian-Gaussian; neural networks; receptive field; 低抗回路網モデル

The purpose of this project was to elucidate the function of retinal neuronal network from the physiological and computational point of views. The following results were obtained in this project. 1).A linear analog network model was proposed to describe the neuronal circuit of the outer retina. 2).Using the model, we characterized the spatial filtering properties of the circuit in terms of the standard regularization theory, in which the early vision problems are attributed to minimization of a cost function. 3).A optical stimulator system was designed to measure the response properties of amacrine cells. The intracellular recordings were obtained from amacrine cells. A relation between the receptive field properties and the morphology of amacrine cells has been studied. 4).The intracellular concentration of Ca^<2+> was measured using fura-2 ratiometric method in isolated horizontal cells to study the Ca^<2+> regulation mechanism. 5).Two pathways of Ca^<2+> influx, i.e. the voltage-gated Ca^<2+> channels as well as the glutamate-gated channels were found. 6).The Na^+ -Ca^<2+> exchange and Ca^<2+> -ATP pump were found to extrude the intracellular Ca^<2+>.7).The results obtained in 5 and 6 suggested that the Ca^<2+> extrusion systems are also play important roles in shaping the light-evoked response in the horizontal cell. A computational study is now undertaken to elucidate this point.

本研究的目的是从生理和计算的角度阐明视网膜神经元网络的功能。本项目取得了以下成果。1）.提出了一种描述视网膜外层神经元回路的线性模拟网络模型。2).使用该模型，我们的特点是在标准的正则化理论，其中的早期视觉问题是由于成本函数的最小化的电路的空间滤波特性。3）设计了一套光刺激系统，用于测量无长突细胞的反应特性。细胞内记录来自无长突细胞。本文研究了无长突细胞的感受野特性与其形态的关系。4).用Fura-2比值法测定离体水平细胞内Ca^2+浓度，探讨Ca^2+调节机制。5). Ca^<2+>内流的两条途径，6）Na^+ -Ca^<2 +>交换和Ca^<2+> -ATP泵对细胞内Ca^<2+进行挤压。7）5和6中的结果表明，Ca^<2+-ATP泵对细胞内Ca^<2+进行挤压，而Ca^<2 +-ATP泵对细胞内Ca ^<2+进行挤压。挤出系统在形成水平细胞中的光诱发响应中也起重要作用。现在进行计算研究来阐明这一点。

项目成果

松本隆 他: "ビジョンチップの動向" 光学. 25-5. 258-264 (1996)

Takashi Matsumoto 等人：“视觉芯片的趋势”25-5 (1996)。

松本隆・小林春夫・八木哲也: "ビジョンチップの動向" 光学. 25・5. 258-264 (1996)

Takashi Matsumoto、Haruo Kobayashi、Tetsuya Yagi：“视觉芯片的趋势”25・5 (1996)。

Kobayashi et al: "Light-Adaptive Architectures for Regularization Vision chips" Neural Networks. 8. 87-101 (1995)

Kobayashi 等人：“正则化视觉芯片的光自适应架构”神经网络。

Hayashida,Y et al.: "Ca^<2+> regulation by the Na^+-Ca^<2+> exchanger in retinal hirizoncal alds" Neuroscience Research. (in press).

Hayashida,Y 等人：“Ca^2 调节视网膜水平性 alds 中 Na^-Ca^2> 交换器”神经科学研究。