Conformational Analysis and Molecular Design of Polypeptides for Creating Advanced Biomaterials

用于创建先进生物材料的多肽构象分析和分子设计

• 批准号: 07680959
• 负责人: OKA Masahito
• 金额: $ 1.34万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680959/
• 关键词: proteins; polypeptides; conformation; molecular mechanics; peptide synthesis; circular dicroism; helix; biomaterials; 円偏光二色性

Theoretical conformational analysis was tried for the periodic polypeptides having repetitive amino-acid sequences which are found in many native periodic proteins such as collagen, elastin, bactenecin, gamma-Zein, et al., and it was shown that they form new interesting helical conformations which are supposed to relate to the molecular functions of such periodic proteins. Experimental analysis was also tried for the synthetic periodic polypeptides, and experimental results supporting the theoretical results were obtained. Moreover, theoretical conformational analysis was tried for the super secondary structures constructed by the secondary structures such as alpha-helices, beta-strands, and polyproline-II structures. It was shown that there are many super secondary structures having interesting structural character from the viewpoints designing new useful.

本文对胶原蛋白、弹性蛋白、bactenecin、γ-玉米醇溶蛋白等天然周期蛋白中存在的具有重复氨基酸序列的周期多肽进行了理论构象分析，并显示它们形成了新的有趣的螺旋构象，这些构象被认为与这些周期蛋白的分子功能有关。对合成的周期性多肽进行了实验分析，得到了支持理论结果的实验结果。此外，还尝试对α-螺旋、β-链和聚脯氨酸-II等二级结构构成的超二级结构进行理论构象分析。结果表明，从设计新的有用的角度来看，有许多具有有趣的结构特征的超二级结构。

项目成果

Y.Ishikawa, M.Oka, T.Hayashi, A.Nishinaga: "Theoretical analysis of alpha-helix hairpin structures constructed by two right-handed alpha-helices." Polym.J.28 (1). 86-90 (1996)

Y.Ishikawa、M.Oka、T.Hayashi、A.Nishinaga：“由两个右手 α 螺旋构建的 α 螺旋发夹结构的理论分析。”

Y.Tanaka, K.Nagamoto, H.Sasaki, T.Fujimoto, N.Nishino, and M.Oka: "Four alpha-helix bundle tructure built with alternating D- and L-segments" Tetrahedron Lett.37 (6). 881-884 (1996)

Y.Tanaka、K.Nagamoto、H.Sasaki、T.Fujimoto、N.Nishino 和 M.Oka：“用交替的 D 段和 L 段构建的四个 α 螺旋束结构”Tetrahedron Lett.37 (6)。

Y.Ishikawa,M.Oka,T.Hayashi,A.Nishinaga,: "Theoretical analysis of α-helix hairpin structures constructed by two right-handed α-helices." Polym.J.28(1). 86-90 (1996)

Y.Ishikawa、M.Oka、T.Hayashi、A.Nishinaga，：“由两个右手 α 螺旋构建的 α 螺旋发夹结构的理论分析。”J.28(1)（ 1996）

M.Oka and T.Hayashi: "Six-β-Strand Bundle Structure;a New Super Secondary Structure Constructed by Anti-Parallel Packing of Six β-Strands" Polymer Journal. 28. 95-97 (1996)

M.Oka 和 T.Hayashi：“六-β-链束结构；由六个 β-链反平行堆积构建的新超级二级结构”聚合物杂志 28. 95-97 (1996)。

Y.Ishikawa,M.Oka,T.Hayashi,and A.Nishinaga: "Theoretical Analysis of α-Helix Hairpin Structures Constructed by Two Right-Handed α-Helices" Polymer Journal. 28. 86-90 (1996)

Y. Ishikawa、M. Oka、T. Hayashi 和 A. Nishinaga：“两个右手 α-螺旋构造的 α-螺旋发夹结构的理论分析”聚合物杂志 28. 86-90 (1996)。