卵巣未熟奇形腫の細胞生物学的分析:組織学的類似性を示す子宮癌肉腫との比較検討

卵巢未成熟畸胎瘤的细胞生物学分析：与子宫癌肉瘤的比较研究显示组织学相似性

• 批准号: 07771417
• 负责人: 江本 精
• 金额: $ 0.7万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07771417/
• 关键词: 卵巣; 未熟奇形腫; 染色体; 子宮; 癌肉腫; 分子生物学; c-myc遺伝子; 組織培養

卵巣未熟奇形腫の患者2名の初回手術時に採取した新鮮腫瘍組織を用いて組織培養を行なった.その結果,細胞株を1種樹立することに成功し,FU-IT-1と命名した.FU-IT-1株は32回の継代に成功し,その細胞形態はspindleで重層化を示し,免疫細胞化学染色にてneuron specific enolase(NSE),S-100,gliofibrillary acidic protein(GFAP)のマーカーに陽性を示し,本株の神経原性起源が示唆された.本株の無血清培養を試みたが,株化には到らなかった.また,同腫瘍の染色体分析を行なった結果,クローナルな異常として第10番染色体と第20番染色体のトリソミ-が認められた.第20番染色体のトリソミ-は既に我々が樹立した子宮癌肉腫(MMMT)株の一つ(FU-MMT-3)にも認められており,両腫瘍の細胞遺伝学的共通性が示唆された.分子生物学的分析では,卵巣未熟奇形腫2例中1例にc-myc癌遺伝子の増幅が認められたが,N-mycの増幅は認められなかった.従って,組織学的類似性を示す卵巣未熟奇形腫とMMMTとの間には,細胞遺伝学的な共通点が存在する可能性が本研究によって明らかとなった.

Two patients with oocyte immature oddball were selected for new tissue culture during the first return to surgery. The results showed that the cell strain was successfully identified by one standing strain, and the FU-IT-1 named strain Fu-IT-1 was successfully subcultured in 32 passages. The cellular spindle of the strain was reconstructed successfully, the immunocytochemical staining showed neuron specific enolase (NSE), Smur100 was identified by gliofibrillary acidic protein (GFAP), and the origin of myogenicity of this strain was instigated. The serum of this strain was cultured, and the strain was isolated from serum to serum. The result of chromosome analysis is the same as that of chromosome analysis. the results show that chromosome 10, chromosome 20, chromosome On the 20th chromosome, the commonness of MMMT (FU-MMT-3) indicates that the commonality of cytology indicates that there is no evidence of cancer. According to the analysis of molecular biology, in 2 cases of immature odd shape of oocyte, 1 case of c-myc cancer showed that the size of the tumor was abnormal, and that of N-myc was the size of the tumor. The similarity of histology shows that there is a possibility that there is something in common in the unripe MMMT of oocytes, and there is a possibility that there is a possibility in this study.