Studies on the pathogenesis and transmission of dengue hemorrhargic fever

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Field studies on the "Pathogenesis and transmission of dengue hemorrhgic fever" in Nakhon Phanom, Northeast Thailand, one of the dengue endemic areas, gave following results :(1) IgM-ELISA is an approprioate method for laboratory confirmation of dengue virus infection, if an ELISA reader and some key reagents (dengue antigen and enzyme-labelled antibodies) were provided. While, RT-PCR is not suitable for routine diagnosis in remote areas where laboratory conditions have not been established. Virus isolation is essential for further detailed analytical studies.(2) Dengue virus type 1,2, and 3 strains were isolated from patient's blood specimens, confirming that Nakhon Phanom is stil one of the dengue-hyperendemic areas.(3) Among several cyotkines examined, IFN-gamma level was elevated more frequently in dengue patients, even in the acute phase, than FUO patients.(4) Patients clinically diagnosed as dengue during dry season are probably infected with other agents.(5) Many female adult Aedes aegypti were captured both in dry and rainy seasons, and most of them were black subspecies with abdominal dorsal tergal pattern 1. Unfortuantely, dengue virus genome was not detected in any of these mosquitoes, leaving the answer for virus susceptibility and hiding places of these mosquiatoes in future studies.(6) Olyset net was selectively applied to those hiding places of Aedes aegypti, which were identified by the studies in dry season. Since such a selective application reduced the number of dengue vector mosquitoes, the method can be utilized for the effective control of dengue with limited amount of resources