Studies on the pathogenesis and transmission of dengue hemorrhargic fever

登革出血热发病机制及传播途径研究

• 批准号: 08041180
• 负责人: IGARASHI Akira
• 金额: $ 4.16万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08041180/
• 关键词: Dengue; Hemorrhagic fever; Cytokines; Variation in vector mosqitoes; Transmission; IgM-ELISA; Vector control; RT-PCR; IgM-ELISA; デング出血熱; 発病機構; 媒介蚊; ウイルス分離; 血清診断; ウイルス遺伝子

Field studies on the "Pathogenesis and transmission of dengue hemorrhgic fever" in Nakhon Phanom, Northeast Thailand, one of the dengue endemic areas, gave following results :(1) IgM-ELISA is an approprioate method for laboratory confirmation of dengue virus infection, if an ELISA reader and some key reagents (dengue antigen and enzyme-labelled antibodies) were provided. While, RT-PCR is not suitable for routine diagnosis in remote areas where laboratory conditions have not been established. Virus isolation is essential for further detailed analytical studies.(2) Dengue virus type 1,2, and 3 strains were isolated from patient's blood specimens, confirming that Nakhon Phanom is stil one of the dengue-hyperendemic areas.(3) Among several cyotkines examined, IFN-gamma level was elevated more frequently in dengue patients, even in the acute phase, than FUO patients.(4) Patients clinically diagnosed as dengue during dry season are probably infected with other agents.(5) Many female adult Aedes aegypti were captured both in dry and rainy seasons, and most of them were black subspecies with abdominal dorsal tergal pattern 1. Unfortuantely, dengue virus genome was not detected in any of these mosquitoes, leaving the answer for virus susceptibility and hiding places of these mosquiatoes in future studies.(6) Olyset net was selectively applied to those hiding places of Aedes aegypti, which were identified by the studies in dry season. Since such a selective application reduced the number of dengue vector mosquitoes, the method can be utilized for the effective control of dengue with limited amount of resources

在登革流行区之一的泰国东北部Nakhon帕诺姆进行的“登革出血热的发病机制和传播”的现场研究表明：（1）如果提供ELISA读数器和一些关键试剂（登革抗原和酶标抗体），IgM-ELISA是实验室确认登革病毒感染的合适方法。而RT-PCR不适合在实验室条件不具备的偏远地区进行常规诊断。病毒分离对于进一步的详细分析研究至关重要。(2)从患者血液标本中分离出1、2、3型登革病毒株，证实那空帕诺姆仍是登革高流行区之一。(3)在检查的几种细胞因子中，IFN-γ水平在登革热患者中更频繁地升高，即使在急性期，也比FUO患者更频繁。(4)在旱季临床诊断为登革热的患者可能感染了其他病原体。(5)在旱季和雨季均捕获到大量埃及伊蚊雌成蚊，且多为黑色亚种，腹背背背纹1型。但在这些蚊体内均未检测到登革病毒基因组，有关这些蚊对登革病毒的易感性及病毒的藏身之处有待进一步研究。(6)在干季，对经调查确定的埃及伊蚊藏匿地点，有选择地使用Olyset网。由于这种选择性施用减少了登革热媒介蚊子的数量，因此该方法可用于在有限量的资源下有效控制登革热

项目成果

江下 優樹: "日本を取り巻く昆虫・ダニ媒介性疾病を考える:デング熱" 生活と環境. 42. 19-22 (1997)

Yuki Eshita：“思考日本周边的昆虫和蜱传疾病：登革热”生活方式与环境。 42. 19-22 (1997)

Itoh T, Okuno T: "Evaluation of the polyethylene net incorporated with permethrin during manufacture of thread on efficacy against Aedes aegypti(Linnaeus)" Medical Entomology and Zoology. 447. 171-174 (1996)

Itoh T、Okuno T：“在线制造过程中掺入氯菊酯的聚乙烯网对埃及伊蚊（林奈）功效的评估”医学昆虫学和动物学。

Eshita Y,Yamada K: "Virus-mosquitoes : Vector competence of Aedes mosquitoes" Antibiotics & Chemotherapy. 12. 2023-2030 (1996)

Eshita Y、Yamada K：“病毒蚊子：伊蚊的媒介能力”抗生素

Itoh,T,Okuno T: "Evaluation of the polyethylene net incorporated with permethrin during maunfacture of thread on efficacy against Aedes aegypti(Linnaeus)" Medical Entomology and Zoology. 447. 171-174 (1996)

Itoh，T，Okuno T：“在线制造过程中掺入氯菊酯的聚乙烯网对埃及伊蚊（林奈）功效的评估”医学昆虫学和动物学。

Itoh T,Okuno T: "Evaluation of the polyethylene net incorporated with permethrin during manufacture of thread on efficacy against Aedes aegypti (Linnaeus)" Med.Entomol Zool. 447. 171-174 (1996)

Itoh T、Okuno T：“在线制造过程中掺入氯菊酯的聚乙烯网对埃及伊蚊（林奈）功效的评估”Med.Entomol Zool。