Epidemiological analysis of dengue virus in the Philippines

采用1995年以来保存在圣卢克医学中心的血清样本，对菲律宾马尼拉的DF/DHF进行了回顾性的血清流行病学研究。所有血清型登革热病毒均在马尼拉流行，每隔两年暴发一次。应用黄病毒交叉反应性单抗(FITC-6B6C-1)建立了一种基于流式细胞术(FCM)的快速、灵敏的登革病毒定量检测方法。用FITC-6B6C-1和藻红蛋白(PE)标记的抗人白细胞表面标志(CD3、CD14、CD16、CD19)对登革热患者外周血白细胞(PBL)进行流式细胞术检测，以确定血液中登革病毒的靶细胞。收集登革热患者的临床资料，如血常规(Ht、抗血小板抗体、RBC、WBC和血小板计数)、胸部X线片和DIC评分，以确定DF和DHF患者的特征。此外，还检测了登革热患者血清中几种细胞因子的水平，以确定与疾病严重程度相关的因素。另一方面，1998年8月至1999年7月，在东多地区(城市模式)和洛斯巴诺斯地区(农村模式)进行了登革热病毒媒介的生态研究。

Seroepidemiological study was performed retrospectively on DF/DHF in Manila, the Philippines using serum samples which were kept at St. Luke's Medical Center since 1995. All serotypes of dengue viruses were prevalent in Manila and outbreaks occurred in 2 year-intervals. A rapid and sensitive quantitative assay for dengue virus based on flow cytometry (FCM) was developed using flavivirus cross reactive monoclonal antibody (FITC-6B6C-1). Dengue patient's peripheral blood leukocyte (PBL) were examined by FCM using FITC-6B6C-1 and phycoerythrin(PE) labeled anti-human leukocyte surface markers (CD3, CD14, CD16, CD19) to determine the dengue virus target cells in the blood. Clinical data of the dengue patients were collected such as blood test (Ht, anti-platelet antibody, RBC, WBC and platelet count), chest X-ray and DIC score to characterize DF and DHF patients. Besides, several cytokine levels were measured in dengue patients' sera to determine the factor related to disease severity. On the other hand, ecological studies were conducted on dengue virus vectors using ovi-traps in Tondo area (as an urban model) and Los Banos area (as a rural model) from Aug. 1998 to Jul. 1999.