Role of myosin-binding poteins in myofibrillogenesis

肌球蛋白结合蛋白在肌原纤维发生中的作用

基本信息

  • 批准号:
    08044191
  • 负责人:
  • 金额:
    $ 4.86万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for international Scientific Research
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1998
  • 项目状态:
    已结题

项目摘要

C-protein is a major myosin-binding protein characteristic of striated muscles. In order to clarity the roles of C-protein in myofibrillogenesis, we carried out the following experiments and obtained the results as described.1) Mouse C-protein cDNA sequences and C-protein isoform expressionThe cDNAs encoding mouse fast skeletal (F) and slow skeletal (S) C-proteins were cloned and their entire sequences were determined. Cardiac (C)-isoform is known to be expressed first and transiently during development of chicken skeletal muscles, but in mouse skeletal muscle, C-isoform was not detected through developmental stages. S-isoform became detectable first, followed by appearance of F-isoform, In the degenerating mouse skeletal muscles, expression of S-isoform is increased.2) Two isoforms in chicken cardiac C-protein and their expressionWe detected two variants (type I and type II) in chicken cardiac C-protein and determined their entire sequences ; they differ only by the inclusion or exclu … More sion of a sequence of 15 amino acid residues (P-seq) which includes a phosphorylation site for A-kinase. We prepared the antibody specific for P-seq. By immunocytochemical methods with this and the other monoclonal antibody to cardiac C-protein (C-315), we found that two isoforms were coexpressed and colocalized in both atrial and ventricular muscles through development, but type I was scarcely expressed in embryonic skeletal muscle. These observations were confirmed by a RT-PCR method.3) Competition of C-protein (MyBP-C) with H-protein (MyBP-H) for binding to myosin in cardiac muscle cellsC-Protein and H-protein are myosin-binding proteins which localize in A-band regions of sarcomeres. To clarify functional relationship of these two myosin binding proteins, we introduced cDNAs encoding these proteins into primary cultures of chicken myocytes and examined their assembly into myofibrils. The results obtained indicate that overexpression of MyBP-C or MyBP-H in cultured cardiac myocytes can reduce the content of MyBP-H or MyBP-C, respectively. These data indicate that MyBP-H can compete with MyBP-C for binding to myosin in the cells. Less
c蛋白是横纹肌的一种主要肌球蛋白结合蛋白。为了明确c蛋白在肌原纤维形成中的作用,我们进行了如下实验,得到如下结果。1)小鼠c -蛋白cDNA序列及c -蛋白异构体表达克隆小鼠快骨骼(F)和慢骨骼(S) c -蛋白编码cDNA,并测定其全序列。心脏(C)-异构体在鸡骨骼肌发育过程中首先短暂表达,但在小鼠骨骼肌中,C-异构体在发育阶段均未被检测到。s -异构体首先被检测到,其次是f -异构体的出现,在退化的小鼠骨骼肌中,s -异构体的表达增加。2)鸡心脏c蛋白的两种异构体及其表达我们检测到鸡心脏c蛋白的两种变异(I型和II型),并测定了它们的全序列;它们的区别只是包含或排除了一个包含a激酶磷酸化位点的15个氨基酸残基序列(P-seq)。制备了P-seq特异性抗体。通过免疫细胞化学方法,我们发现该蛋白和另一种心脏c蛋白(C-315)单克隆抗体在发育过程中在心房肌和心室肌中共表达和共定位,但I型在胚胎骨骼肌中几乎不表达。这些观察结果被RT-PCR方法证实。3)心肌细胞中c蛋白(MyBP-C)与h蛋白(MyBP-H)结合肌球蛋白的竞争。c蛋白和h蛋白是肌球蛋白结合蛋白,定位于肌节的a带区。为了阐明这两种肌球蛋白结合蛋白的功能关系,我们将编码这两种蛋白的cdna引入鸡肌细胞的原代培养中,并检测了它们在肌原纤维中的组装。结果表明,培养心肌细胞过表达MyBP-C或MyBP-H可分别降低MyBP-H或MyBP-C的含量。这些数据表明MyBP-H可以在细胞中与MyBP-C竞争与肌球蛋白的结合。少

项目成果

期刊论文数量(44)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hayakawa,Kimihide: "Differential assembly of cytoskeletal and sarcomeric actins in developing skeletal muscle cells in vitro." Zool. Sci.13. 509-517 (1996)
Hayakawa,Kimihide:“体外发育骨骼肌细胞时细胞骨架和肌节肌动蛋白的差异组装。”
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  • 影响因子:
    0
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  • 通讯作者:
Nagaoka, Rie: "Quantitative and analysis of low MW-actin-binding proteins, cofilin, ADF and profilin, expressed in developing and degenerating chicken skeleta muscles." J.Muscle Res Cell Moeil.17. 463-473 (1996)
Nagaoka, Rie:“对在发育和退化的鸡骨骼肌中表达的低分子量肌动蛋白结合蛋白、肌动蛋白丝切蛋白、ADF 和 profilin 进行定量和分析。”
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  • 影响因子:
    0
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  • 通讯作者:
Obinata, Takashi: "Low molecular-waight G-actin binding proteins mvolved in the regulation of actin assembly during myofibrillogenesis." Cell. Str. Func.22(1). 181-189 (1997)
Obinata, Takashi:“低分子量 G 肌动蛋白结合蛋白参与肌原纤维形成过程中肌动蛋白组装的调节。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Vohra, M.S.: "Subcellular localization of dystrophin and vinculin in cardiac muscle fibers of the conduction system of the chicken ventricle." Cell Tiss. Res.294. 137-143 (1998)
Vohra,M.S.:“鸡心室传导系统心肌纤维中肌营养不良蛋白和纽蛋白的亚细胞定位。”
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  • 影响因子:
    0
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Soeno, Yoshinori: "BDM (2,3-Butanedione monoxime), an inhibitor for myosin-actin interaction, suppresses myofibrillogenesis in skeletal muscle cells in culture." Cell Tiss. Res.295. 307-316 (1999)
Soeno,Yoshinori:“BDM(2,3-丁二酮单肟)是一种肌球蛋白-肌动蛋白相互作用的抑制剂,可抑制培养的骨骼肌细胞中的肌原纤维形成。”
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  • 影响因子:
    0
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OBINATA Takashi其他文献

OBINATA Takashi的其他文献

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{{ truncateString('OBINATA Takashi', 18)}}的其他基金

Regulation of cell motility by troponin, a calcium-regulatory protein.
通过肌钙蛋白(一种钙调节蛋白)调节细胞运动。
  • 批准号:
    23570097
  • 财政年份:
    2011
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
An Inventory of Financial Accounting Research in Japan : Under the Changing Circumstances on Accounting Research
日本财务会计研究盘点:变迁下的会计研究
  • 批准号:
    21330109
  • 财政年份:
    2009
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Functional role of troponin, a muscle regulatory protein, in non-striated muscle
肌钙蛋白(一种肌肉调节蛋白)在非横纹肌中的功能作用
  • 批准号:
    20570075
  • 财政年份:
    2008
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Functional variation of troponin, a muscle regulatory protein, during evolution of chordate animals
脊索动物进化过程中肌钙蛋白(一种肌肉调节蛋白)的功能变化
  • 批准号:
    17370025
  • 财政年份:
    2005
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The Rate Regulation and the Relationship between Stock prices and Earnings
利率管制与股价与盈利的关系
  • 批准号:
    15530297
  • 财政年份:
    2003
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Functional properties of cofilin and C-protein molecules and their roles in formation and maintenance of muscle cellular structure
丝切蛋白和 C 蛋白分子的功能特性及其在肌肉细胞结构形成和维持中的作用
  • 批准号:
    14340262
  • 财政年份:
    2002
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of formation and maintenance of muscle contractile apparatus (myofibrils)
肌肉收缩装置(肌原纤维)形成和维持的分子机制
  • 批准号:
    10304065
  • 财政年份:
    1998
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
A cell-stretching system as a model system for the study of biomechanics
细胞拉伸系统作为生物力学研究的模型系统
  • 批准号:
    08558078
  • 财政年份:
    1996
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Studies on molecular structure of C-protein and its role in myofibrillogensis
C蛋白分子结构及其在肌原纤维形成中的作用研究
  • 批准号:
    06454024
  • 财政年份:
    1994
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Actin dynamics and the roles of ADF and cofilin : studies with muscle and nerve as model systems
肌动蛋白动力学以及 ADF 和丝切蛋白的作用:以肌肉和神经为模型系统的研究
  • 批准号:
    05044122
  • 财政年份:
    1993
  • 资助金额:
    $ 4.86万
  • 项目类别:
    Grant-in-Aid for international Scientific Research

相似海外基金

Function and structure of the spike H protein of bacteriophage φX174 directing infection to host cell
噬菌体φX174刺突H蛋白引导感染宿主细胞的功能和结构
  • 批准号:
    17510177
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Establishment and analyses of mouse models of glycine-encephalopathy by conditional knockout of mouse H protein-gene.
通过条件性敲除小鼠H蛋白基因建立小鼠甘氨酸脑病模型并进行分析。
  • 批准号:
    14370052
  • 财政年份:
    2002
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    $ 4.86万
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BIOPHYSICAL CHEMISTRY OF A DEAD/H PROTEIN
死/H 蛋白的生物物理化学
  • 批准号:
    6387041
  • 财政年份:
    2000
  • 资助金额:
    $ 4.86万
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Biophysical Chemistry of a DEAD/H Protein
DEAD/H 蛋白的生物物理化学
  • 批准号:
    6827704
  • 财政年份:
    2000
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    $ 4.86万
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Biophysical Chemistry of a DEAD/H Protein
DEAD/H 蛋白的生物物理化学
  • 批准号:
    7102735
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    2000
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BIOPHYSICAL CHEMISTRY OF A DEAD/H PROTEIN
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    $ 4.86万
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BIOPHYSICAL CHEMISTRY OF A DEAD/H PROTEIN
死/H 蛋白的生物物理化学
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    6636362
  • 财政年份:
    2000
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BIOPHYSICAL CHEMISTRY OF A DEAD/H PROTEIN
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    6195779
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    2000
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Biophysical Chemistry of a DEAD/H Protein
DEAD/H 蛋白的生物物理化学
  • 批准号:
    7263029
  • 财政年份:
    2000
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Biophysical Chemistry of a DEAD/H Protein
DEAD/H 蛋白的生物物理化学
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    6930466
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