Joint study on the mechanism of recognition in corals

珊瑚识别机制联合研究

• 批准号: 08044216
• 负责人: HIDAKA Michio
• 金额: $ 8.06万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044216/
• 关键词: coral; self and nonself recognition; contact reaction; symbiosis; zooxanthellae; symbiont-host specificity; 自己・非自己認識; 組織適合性; 種間相互作用; 組織非適合; イシサンゴ; 自他認識

We showed that contact reaction between young colonies of the coral Pocillopora damicornis changes. Someinitially fused pairs later showedincompatible fusion and then nonfusion. Many vacuoles and condensed nucleiwere observed at the interface of histoincompatible pairs suggesting that celldeath through apoptosis occurred.Corals belonging to different families did not always compete through extracoelenteric digestion. Though presence of coelenteric digestion indicates that the two corals belong to different species, absenc of coelenteric digestiondoes not mean the two corals are phylogenetically related.Yolk protein of five scieractinian corals were analyzed biochemically and immunologicaIIy. Yolk proteins of congeneric corals were similar to each otherwhile those from corals belonging to different families differ significantly.Abnormal growths (tumors) in Porites lobata and P.compressa showed skeletaimorphology different from normal polyp and reduced reproductive output. However, squashes of coichicine-treated blastulae obtained from crosses using gametes from tumors and mormal polyps revealed similarnumber of chromosomes.

我们发现，年轻的珊瑚Pocillopora damicornis的变化殖民地之间的接触反应。一些最初融合的对后来表现出不相容的融合，然后不融合。在组织不相容对的界面上观察到许多空泡和浓缩的细胞核，表明细胞通过凋亡而死亡。虽然存在体腔消化表明这两种珊瑚属于不同的种，但不存在体腔消化并不意味着这两种珊瑚在遗传上有亲缘关系。同种珊瑚的卵黄蛋白质相似，不同科珊瑚的卵黄蛋白质差异显著，裂叶滨珊瑚和扁滨珊瑚的异常生长（肿瘤）表现出与正常珊瑚不同的骨骼形态，生殖输出减少。然而，从肿瘤和正常息肉的配子杂交中获得的经胭脂虫碱处理的囊胚的挤压物显示出相似的染色体数目。

项目成果

Hidaka M.,K,Yurugi, S.Sunagawa, R.A.Kinzie III: "Contact reactions between young colonies of the coral Pocillopora damicornis." Coral Reefs. 16. 13-20 (1997)

Hidaka M.,K,Yurugi, S.Sunakawa, R.A.Kinzie III：“珊瑚 Pocillopora damicornis 的年轻群体之间的接触反应。”

Kinzie RA III and RW Buddemeier: "Reefs Happen." Global Change Biology. 2. 479-494 (1996)

Kinzie RA III 和 RW Buddemeier：“珊瑚礁发生了。”

Sakai K: "Effect of colony size, polyp size and budding mode on eggproduction in a colonial coral." Biological Bulletin. 195. 319-325 (1998)

Sakai K：“群体大小、息肉大小和出芽模式对殖民珊瑚产卵的影响。”

Kinzie RA III: "Modes of speciation and reproduction in Archaeocoeniid corals." Galaxea. 13・1. 47-64 (1996)

Kinzie RA III：“太古珊瑚的物种形成和繁殖模式。”13・1（1996）。

Sakai K: "Delayed maturation in the colonial coral Goniastrea aspera (Scleractinia) : Whole-colony mortality, colony growth and polyp egg production." Researches on Population Ecology. 40. 287-292 (1998)

Sakai K：“群落珊瑚 Goniastrea aspera（Scleractinia）的延迟成熟：整个群落死亡率、群落生长和息肉卵的产生。”