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DEVELOPMENT AND GENETIC CONTROL OF HISTOINCOMPATIBILITY IN SCLERACTINIAN CORALS.

石珊瑚组织不相容性的发育和遗传控制。

基本信息

批准号：
04804063
负责人：
HIDAKA Michio
金额：
$ 1.02万
依托单位：
UNIVERSITY OF THE RYUKYUS
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04804063/
关键词：
CORALS HISTOCOMPATIBILITY CHIMERA ALLOZYME サンゴ自他認識刺胞動物

项目摘要

Contact responses between primary polyps or young colonies derived from different colonies of the coral Pocillopora damicornis were examined. Polyps from the same colonies fused with each other. Polyps from different colonies showed either fusion, nonfusion, or incompatible fusion. In non-fused pairs, tissue was discontinuous and a skeletal ridge was formed at the interface. In incompatible fusion, tissues of the paired polyps appeared continuous but a white zone without zooxanthellae was observed at the interface of the polyps. Newly formed polyps located near the white zone sometimes disappeared or were partially resorbed as the white zone widened. When the skeleton of the polyps was observed with a SEM, the skeleton was continuous but a groove with skeletal spines on both sides was observed at the interface. Most of the polyps which showed incompatible fusion became separated by a skeletal ridge as in non-fused pairs or by a narrow zone of skeleton without living tissue after 2 - 8 months. The incompatible fusion might be a histoincompatible response which may later transform into nonfusion response.Polyps isolated from different colonies of Galaxa fascicularis were brought into contact. Polyps isolated from colonies belonging to different color morphs generally did not fuse with each other. However, in one combination (Gt-Gs), Tissue appeared continuous but a white zone with no or only a few zooxanthellae was observed at the interface. To examine genetic nature of the chimeric colonies produced by fusion of polyps belonging to different coloe morphs, I attempted to examine the allozyme pattern of constituents of the chimeric colonies. Eighteen enzymes were stained but only 8 enzymes produced interpretable bands. Since constituents of the chimeric colonies displayd the same zymographs, I could not analyze the genetic nature of the chimeras.

初级息肉或来自不同的珊瑚Pocillopora damicornis殖民地的年轻殖民地之间的接触反应进行了检查。来自同一菌落的息肉彼此融合。来自不同菌落的息肉显示融合、不融合或不相容融合。在非融合对中，组织是不连续的，并且在界面处形成骨骼嵴。在不相容融合中，成对息肉的组织呈现连续性，但在息肉界面处观察到无虫黄嘌呤的白色区。当白色区变宽时，位于白色区附近的新形成的息肉有时消失或部分吸收。当用SEM观察息肉的骨架时，骨架是连续的，但在界面处观察到两侧具有骨架棘的凹槽。大多数显示不相容融合的息肉在2 - 8个月后被骨骼脊分开，如在非融合对中或被没有活组织的狭窄骨骼区分开。这种不相容的融合反应可能是一种组织不相容的反应，以后可能转化为非融合反应。从属于不同颜色变体的菌落分离的息肉通常不会彼此融合。然而，在一种组合（Gt-Gs）中，组织看起来是连续的，但在界面处观察到一个白色区，没有或只有少量虫黄嘌呤。为了研究由属于不同coloe变体的息肉融合产生的嵌合菌落的遗传性质，我试图研究嵌合菌落的成分的等位酶模式。18种酶被染色，但只有8种酶产生可解释的条带。由于嵌合体菌落的成分显示出相同的酶谱，我无法分析嵌合体的遗传特性。