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Mechanisms of absorption, transport, and storge of vitamin A-by using rats and polar animals-

维生素A的吸收、运输和储存机制-利用大鼠和极地动物-

基本信息

批准号：
08044237
负责人：
SENOO Haruki
金额：
$ 1.34万
依托单位：
Akita university School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044237/
关键词：
Svalbard archipelago Arctic animal vitamin A hepatic stellate cell hypervitaminosis A leukemin cancer therapy retionid-binding proteins

项目摘要

Seven Svalbard reindeers, 3 ringed seals, 2 bearded seals, 4 glaucous gulls, 3 Brunnich's guillemots, and 1 puffin were caught in the Svalbard archipelago from August through September, 1996. Fresh each organs, namely, the liver, kidneys, lungs, jejunum, spleen, and skin were investigated with gold chloride staining, fluorescence microscopy for autofluorescence of vitamin A,Sudan 3 lipid staining, hematoxylin and eosin staining, Azan staining, Ishii and Ishii's silver impregnation method, and transmission electron microscopy.The Arctic animals and birds store a large amount of vitamin A in hepatic stellate cells. Only a small amount of vitamin A existed in other organs : kidneys, jejunum, spleen, lungs, and skin. On the other hand, extrahepatic stellate cells in normal rats were demonstrated to store vitamin A in the kidneys, lungs, spleen, and intestine.These results indicated that the Arctic animals and birds can store a huge amount of vitamin A in hepatic stellate cells in physiological conditions. These data also indicated that the molecular mechanisms such as retinoid-binding proteins in the Arctic animals and birds play pivotal roles in their vitamin A storage. Applying these molecular mechanisms the prevention methods will be developed for hypervitaminosis A as a side effect of vitamin A treatment to malignant diseases such as leukemia.

1996年8月至9月，在斯瓦尔巴群岛捕获了7只斯瓦尔巴驯鹿、3只环斑海豹、2只胡须海豹、4只苍鸥、3只布伦尼奇海鸥和1只海雀。对新鲜的肝、肾、肺、空肠、脾和皮肤进行了氯化金染色、维生素A自体荧光荧光显微镜、苏丹红3脂染色、苏木精伊红染色、Azan染色、Ishii和Ishii‘s银染法和透射电子显微镜观察。只有少量的维生素A存在于其他器官中：肾脏、空肠、脾、肺和皮肤。另一方面，正常大鼠的肝外星状细胞在肾、肺、脾和肠道中储存维生素A，这一结果表明北极动物和鸟类在生理条件下可以在肝星状细胞中储存大量维生素A。这些数据还表明，北极动物和鸟类中的维甲酸结合蛋白等分子机制在它们的维生素A储存中起着关键作用。应用这些分子机制，将开发出预防维生素A过多症的方法，这是维生素A治疗对白血病等恶性疾病的副作用。