Mechanisms of absorption, transport, and storage of vitamin A-by using rats and polar animals-

维生素A的吸收、运输和储存机制-利用大鼠和极地动物-

• 批准号: 09044251
• 负责人: SENOO Haruki
• 金额: $ 4.42万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044251/
• 关键词: vitamin A; Arctic animals; hepatic stellate cells; retinyl ester; Svalbard archipelago; hypervitaminosis A; food web; hepatic fibrosis; レエチニルエステル; スバルバ-ル群島; 自家蛍光; 塩化金法

Two polar bears, 8 Arctic foxes, 6 bearded seals, 22 glaucous gulls, 5 fulmars, 4 Bruenich's guillemots, 6 ringed seals, 5 hooded seals, 6 puffins, and 7 Svalbard reindeers were caught in the Svalbard archipelago (situated at lat. 80 N). Fresh organs, namely, the liver, kidney, spleen, lung, and jejunum were investigated with gold chloride staining, fluorescence microscopy for autofluorescence of vitamin A, hematoxylin and eosin staining, azan staining, Ishii-Ishii's silver impregnation, transmission electron microscopy and high-performance liquid chromatography.The mammals and birds stored a large amount of vitamin A in the liver [23,300 (polar bear, n=2), 18,439*2,314 (Arctic fox, n=8), 5956*5857 (bearded seal, n=6), 1,551*2,486 (ringed seal, n=6), 921*264 (Svalbard reindeer, n=7), 4,710*3,164 (glaucous gull, n=22), 1,589*225 (Bruenich's guiHemot, n=4), and 1183*589 (puffin, n=6) nmole retinyl ester/g wet tissue]. Very strong autofluorescence of vitamin A emanated from the non-parenchymal cells in the liver. These cells showed positive reaction with gold chloride and stained black. Electronmicroscopically these cells existed in the perisinusoidal space of Disse and contained many lipid droplets within the cytoplasm. These anamals showed no hepatic fibrosis though they are in naturally occurred hypervitaminosis A.These findings suggest that these vitamin A-storihg cells are hepatic stellate cells and the Arctic animals that exist in a higher position of the food web in the Arctic area have well-developed vitamin A-storing mechanisms in the hepatic stellate cells.

在斯瓦尔巴群岛（位于北纬100度）捕获了2只北极熊、8只北极狐、6只须海豹、22只海鸥、5只管鼻蝠、4只布鲁尼希海鸠、6只环斑海豹、5只冠海豹、6只海雀和7只斯瓦尔巴驯鹿。80 N）。用氯化金染色、维生素A自发荧光显微镜、苏木精-伊红染色、azan染色、Ishii-Ishii's银浸渍、透射电子显微镜和高效液相色谱法研究了新鲜器官，即肝、肾、脾、肺和空肠。哺乳动物和鸟类在肝脏中储存了大量的维生素A [23，300（北极熊，n=2），18，439 * 2，314（北极狐，n=8），5956*5857（须海豹，n=6），1,551* 2486（环形密封，n=6），921*264（斯瓦尔巴驯鹿，n=7）、4，710 * 3，164（银鸥，n=22）、1，589 *225（布氏鸥，n=4）和1183*589（海雀，n=6）nmole视黄酯/g湿组织]。非常强的维生素A的自发荧光从肝脏中的非实质细胞发出。这些细胞与氯化金反应呈阳性，并被染成黑色。电镜下，这些细胞存在于窦周间隙的Disse和含有许多脂滴的细胞质。这些动物虽然在自然发生的维生素A过多症中没有表现出肝纤维化。这些结果表明，这些维生素A储存细胞是肝星状细胞，并且存在于北极地区食物网较高位置的北极动物在肝星状细胞中具有发达的维生素A储存机制。

项目成果

Senoo H,et al.: "Molecular mechanisms in the reversible regulation of morphology,proliferation,and collagen metabolism in hepatic stellate cells by the three-dimensional structure of ECM" J Gastroent Hepatol. 13. S19-32 (1998)

Senoo H 等人：“ECM 三维结构对肝星状细胞形态、增殖和胶原代谢的可逆调节的分子机制”J Gastroent Hepatol。

Nagy NE,et al.: "Storage of vitamin A in extrahepatic stellate cells in normal rats." J Lpid Res.38. 645-658 (1997)

Nagy NE 等人：“正常大鼠肝外星状细胞中维生素 A 的储存。”

Senoo H,et al.: "Hepatic stellate cells-from viewpoint of retinoid handling and functions of extracellular matrix" Acta Anat Nippon. 72. 79-94 (1997)

Senoo H 等人：“肝星状细胞 - 从类维生素A处理和细胞外基质功能的角度来看”Acta Anat Nippon。

Kojima N,et al.: "Stellate cells (vitamin A-storing cells) change their cytoskeleton and signal transduction system according to extracellular matrix" Histochem Cell Biol. 110. 121-128 (1998)

Kojima N 等人：“星状细胞（维生素 A 储存细胞）根据细胞外基质改变其细胞骨架和信号转导系统”Histochem Cell Biol。

Senoo H, et al.: "Molecular mechanisms in the reversible regulation of morphology, proliferation, and collagen metabolism in hepatic stellate-cells by the three-dimensional structure of the ECM" J Gastroent Hepatol. 13. S19-32 (1998)

Senoo H 等人：“ECM 三维结构对肝星状细胞形态、增殖和胶原代谢进行可逆调节的分子机制”J Gastroent Hepatol。