Immune response at the fetal-maternal interface during normal gestation

1. 采用磁性细胞分离法分离子宫NK细胞，明确了细胞在趋化因子细胞外基质和凋亡诱导因子作用下的动力学。2 .通过添加多种细胞因子/趋化因子，研究了子宫NK细胞祖细胞的体外分化，并特别参考了NK细胞的细胞溶解和细胞质颗粒的形态和性质。CD3e转基因小鼠可用于NK细胞功能的研究。利用该动物模型，观察了VCAM和ICAM对子宫NK细胞血管内皮的相互作用。进一步探讨了子宫NK细胞分化与MHC II类或细胞因子注射所致流产的关系；这阐明了习惯性流产与NK细胞的密切关系。胎儿-母体界面的免疫反应是受精卵着床形成胎盘形成妊娠的一个确定因素。这些研究提供了可能调控植入的体内数据；这些资料不仅有助于弄清习惯性流产的发病机制，而且有助于建立习惯性流产的动物模型。大阪府立大学的一位教授为了继续推进该项目，前往阿根廷（布宜诺斯艾利斯大学），美国（布宜诺斯艾利斯大学）。哈佛大学，加利福尼亚大学)和加拿大（圭尔夫大学）。一些教授被邀请到大阪府立大学，圭尔夫大学。

1. The kinetics of uterine NK cells under chemokine-included extracellular matrix and apoptosis-induced factors was clarified by isolating the cells using magnatic cell isolation methods.2.The differentiation of progenitor cells of uterine NK cells was investigated in vitro by addition of a various kinds of cytokines/chemokines, with special references to cytolysis, and morphology and nature of cytoplamic granules of NK cells.3.CD3e transgenic mice would be useful for investigation of NK cell function. Using this animal model, interactions of VCAM and ICAM on vascular endothelia to uterine NK cells was examined. Furthermore, the relationship between uterine NK cell differentiation and abortion due to uncomparable MHC class II or cytokine injection was investigated ; this clarified close relationship of habitual abortion to NK cells.4.Immune response at the fetal-maternal interface is a definite factor for establishment of gestation through placenta formation from fertilized ovary implantation. These studies provided the in vivo data of possible regulation of implantation ; these data lead not only to clarification of pathogenesis of habitual abortion but also to production of animal models of the habitual abortion.5.In order to continue and promote this project, one professor of Osaka prefecture University went to Argentina (Univ. BuenosAires), U.S.A.(Univ. Harvard, and Univ. California), and Canada (Univ. Guelph). Some professors, Univ. Guelph, were invited to Osaka Prefecture University.