Functional roles of macrophage populations appearing in the renal fibrosis

巨噬细胞功能各异，主要有渗出型、常驻型（组织细胞）和抗原呈递型树突状细胞三大类。本研究探讨了巨噬细胞群的性质及其在肾纤维化中的作用。慢性肾纤维化中巨噬细胞的特征：反复注射具有肾毒性的顺铂所致的慢性肾纤维化中，渗出性和常驻性巨噬细胞早期增多，晚期减少。另一方面，树突状细胞在早期和晚期出现的数量更多。由于晚期常出现淋巴细胞，表达MHCⅱ类的树突状细胞可能与淋巴细胞的诱导有关。牛磺酸对大鼠肾纤维化模型的影响：在顺铂诱导的大鼠肾纤维化中，经抗氧化牛磺酸处理后，巨噬细胞数量减少，导致纤维化减少。硫代乙酰胺（TAA）诱导大鼠肝纤维化的巨噬细胞群特征：为了与巨噬气体在肾纤维化中的作用进行比较，我们研究了TAA诱导的肾纤维化病变，并特别参考了巨噬细胞群。肝纤维化中存在渗出性巨噬细胞和常驻性巨噬细胞以及树突状细胞，但这些巨噬细胞群在出现和分布的时间点上存在差异。与肾纤维化相比，Kupffer细胞似乎在肝纤维化中起重要作用，尽管表达MHC ii类树突状细胞增加，但淋巴细胞数量较少。肾纤维化体外细胞模型的建立：我们试图建立大鼠胸腺源性树突状细胞肉瘤细胞系（KB-D8）。KB-D8可能有助于研究与肾纤维化有关的树突状细胞性质。

Macrophagss show heterogeneous functions, consisting of three major types such as exudate macrophages, resident macrophages (histiocytes), and antigen-presenting dendritic cells. This study investigated the properties and participation of macrophage populations in the renal fibrosis.1. Properties of macrophages appearing in chronic renal fibrosis: In the chronic renal fibrosis, that had been induced by repeated injections of cisplatin with nephrotoxicity, exudate and resident macrophages increased at the early stages, and then decreased at the late stages. On the other hand, dendritic cells appeared in greater number at both early and late stages. Because lymphocytes were often present in the late stagss, MHC class II-expressing dendritic cells may be related to induction of lymphocytes.2. Effects of taurine on rat renal fibrosis model: In cisplatin-induced rat renal fibrosis, that had been treated with taurine with antioxidant properties, macrophage numbers were decreased, resulting in reduced fibrosis.3. Characteristics of macrophage populations in thioacetoamide (TAA)-induced rat hepatic fibrosis: In order to compare with macrophagss in renal fibrosis, we investigated lesions of TAA- induced renal fibrosis, with special reference to macrophage populations. In hepatic fibrosis, exudate and resident macrophages, and dendritic cells were present, but there were differences in time points of appearance and distribution between these macrophage populations. In contrast to renal fibrosis, Kupffer cells seemed to play an important role in hepatic fibrosis, and lymphocytes were smaller in number, even though MHC class II-expressing dendritic cells were increased.4. Establishment of in vitro cell model for renal fibrosis: We attempted to establish a cell line (KB-D8) from rat thymus-derived dendritic cell sarcoma. KB-D8 might be useful for studies on dendritic cell nature relating to renal fibrosis.