Molecular Mechanism of Dynamics of Vacuolar System in Plant Cell

植物细胞液泡系统动力学的分子机制

• 批准号: 08304044
• 负责人: OHSUMI Yoshinori
• 金额: $ 15.81万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08304044/
• 关键词: ER; Golgi Body; Vacuole; Storage Protein; Water Channel; Autophagy; Vesicular Transport; Phosphate Transport; 植物細胞; 酵母; 空胞系; タンパク質のソーティング

One of the most important features of eukaryotic cells is developement of complex intracellular single membrane system called vacuolar system. ER,Golgi body, lysosome and plasma membrane are dynamically connected each other by specific vesicles. In plant cell still not much is known about the molecular machinery for the dynamic aspect of the vesicular trafficing. This research program aimed to elucidate specific features of vacuolar system in plant cells, by comparing various model systems which each group is working with including yeast, Green algi, culture cell and higher plant cell. We could discuss about many different interesting results obtained by each group and several cooperation started during this research program.On the mechanisms of bulk protein degradation (autophagy), protein trafficing for storage protein, vacuolar protein and membrane biogenesis, water channel, many important findings were reported.

真核细胞最重要的特征之一是发育出复杂的胞内单膜系统，称为空泡系统。内质网、高尔基体、溶酶体和质膜通过特定的囊泡相互动态连接。在植物细胞中，关于囊泡运输的动态方面的分子机制仍然知之甚少。本研究计划的目的是通过比较各个研究小组正在研究的各种模型系统（包括酵母、绿色藻类、培养细胞和高等植物细胞）来阐明植物细胞中液泡系统的具体特征。我们可以讨论许多不同的有趣的结果，每个小组和几个合作开始在这个研究计划中，大量的蛋白质降解（自噬），蛋白质运输为存储蛋白，液泡蛋白和膜生物合成的机制，水通道，许多重要的发现报告。

项目成果

Koide, Y.他3名: "The N-terminal propeptide of the precursor to sporamin acts as a vacuolar targeting signal even at the C-terminus of the mature part in tobacco cells." Plant Physiol.114. 863-870 (1997)

Koide, Y. 和其他 3 人：“sporamin 前体的 N 端前肽甚至在烟草细胞成熟部分的 C 端也充当液泡靶向信号。”114 (1997) ）

Mimura, T.他5名: "Efficient callus initiation from leaf of mangrove plant,Bruguiera sexangula in amino acid medium:Effect of NaCI on callus initiation." Journal of Plant Research. 110. 25-29 (1997)

Mimura, T. 和其他 5 人：“氨基酸培养基中红树植物叶的高效愈伤组织起始：NaCI 对愈伤组织起始的影响”，植物研究杂志 110. 25-29 (1997)。

Nose, Y.他5名: "Specific increase in thc number of vanacdium-containing blood cells by some ionophores-ATPase in the ascidian Ascidia sydneiensis samea." Zool.Sci.14. (197)

Nose, Y. 和其他 5 人：“海鞘中某些离子载体-ATP 酶对含钒血细胞数量的特定增加。” (197)。

Shiratake, K.他3名: "Changes in H^+-pumps and a tonoplast intrinsie protein of vacuolar membranes during the development of pear fruit." Plant Cell Physiol.38. 1039-1045 (1997)

Shiratake, K. 和其他 3 人：“梨果实发育过程中 H^+ 泵和液泡膜固有蛋白的变化。”38（1997 年）。

Mimura, T.他2名: "Control of phosphate transport across the plasma membrane of Chara corallina" Journal of Experimental Botany. 49. 13-19 (1998)

Mimura, T. 和其他 2 人：“控制珊瑚藻质膜上的磷酸盐运输”实验植物学杂志 49. 13-19 (1998)。