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Studies on molecular genetics of bacterial pathogenesis

细菌发病机制的分子遗传学研究

基本信息

批准号：
08307004
负责人：
NAKAZAWA Teruko
金额：
$ 14.08万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08307004/
关键词：
Two component regulatory system collagenase motility hemolysin Ipa protein Vibrio genome virulence plasmid PHICTX phage 病原細菌緑膿菌赤痢菌Ipa蛋白 P.gingivalis 腸炎ビブリオ B.fragilis ボツリヌス毒素ヘリコバクター・ピロリ

项目摘要

1 . Colonization and proliferation factors of bacteria1)Genes for two component regulatory systems of P.gingivalis were analyzed (Yoshimura).2)Genes for bacteriocins and their secretion system were identified (Ike).3)Iron-responsive repressor, fur of Vibric and fur-regulated genes were identified (S.Yamamoto).4)H.pylon urease is involved in motility in the stomach (Nakazawa).5)P.aeruginosa fur, dnaJ, and grpE were identified as essential genes (Tsuda).6)Two component regulatory systems in S.aureus and C.perfringens were analyzed (Hayashi).7)Serum resistance gene in Salmonella, pagC, was identified (Danbara)8)New adhesion mechanisms of E.ccli were characterized (T.Yamamoto).2.Factors of host-injury and invasiveness1)Two collagenases from C.historiticum was identified and characterized (Okabe).2)P.gingivalis peptidase was identified (Yoshikawa).3)B.faragilis neuraminidase was analyzed (Onishi).4)NAD-degrading enzyme was identified in group A streptococci (Nakamura).5)Specific binding of HA components of botulinus toxin to intestinal microvilli was shown (Oguma).6)Pore-forming mechanism of S.aureus hemolysin was analyzed (Kamio).7)Induction mechanisms of signal transduction and actin polymerization by S.felxneri Ipa and VirG, respectively, were clarified (Sasakawa).3.Genome analysis1)Two chromosomes of V.parahaemolyticus was identified (Honda).2)Salmonella virulence plasmid (46kb) was sequenced (Danbara).3)PHICTX phage genome was sequenced (Terawaki).

１．细菌的定植和增殖因子1)对牙龈假单胞菌双组分调控系统的基因进行了分析（Yoshimura）。2)确定了细菌素及其分泌系统的基因（Ike）。3)鉴定出铁响应抑制因子、绒毛及绒毛调控基因（S.Yamamoto）。塔脲酶参与胃的运动（Nakazawa）。铜绿假单胞菌（aeruginosa fur）、dnaJ和grpE被确定为必需基因（Tsuda）。6)分析了金黄色葡萄球菌和产气荚膜球菌的两种成分调控系统（Hayashi）。7)沙门氏菌血清耐药基因pagC的鉴定（Danbara）； 8)大肠杆菌黏附新机制的研究（T.Yamamoto）。宿主损伤和侵袭的因素1)鉴定和鉴定了两种历史菌胶原酶（Okabe）。牙龈肽酶鉴定（Yoshikawa）。分析faragilis神经氨酸酶（Onishi）。4)在A组链球菌（Nakamura）中发现了nad1降解酶。5)肉毒杆菌毒素的HA成分与肠道微绒毛的特异性结合（Oguma）。6)分析了金黄色葡萄球菌溶血素的成孔机制（Kamio）。7)阐明了S.felxneri Ipa和VirG分别诱导信号转导和肌动蛋白聚合的机制（Sasakawa）。基因组分析1)鉴定出副溶血性弧菌的两条染色体（Honda）。2)沙门氏菌毒力质粒（46kb）测序（Danbara）。3)PHICTX噬菌体基因组测序（Terawaki）。