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Bioprocess methanol production from methane.

利用甲烷进行生物工艺生产甲醇。

基本信息

批准号：
08455373
负责人：
OKURA Ichiro
金额：
$ 4.74万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Direct oxidation of methane to methanol is a highly attractive process compared to the current process consisting of energy intensive conversion of methane by steam into synthesis gas. Methane monooxygenase (MMO) catalyzes single-step oxidation of methane to methanol. But MMO could not be used in methanol synthesis, because of instability. Thus methanol production from methane was tried with methanotroph. Methylosinus trichosporium OB3b, a methanotrophic bacterium, contains MMO and catalyzes hydroxylation of methane to methanol.When the methane is oxidized, produced methanol is subsequently oxidized by methanol dehydrogenase containing in the same bacterium. To prevent further oxidation of methanol, the cell suspension was treated by cyclopropanol, which was found to be an irreversible inhibitor for methanol dehydrogenase by the previous study, leading to extracellular methanol accumulation. The purpose of this research is to increase methanol production and to improve methanol yield a … More nd the efficiency.The batch type of methanol synthesis by M.trichosporium OB3b is terminated at c.a.6 mM of methanol, because MMO is inhibited by increasing methanol concentration. For prolonged methanol accumulation, a semicontinuous process was carried out in this study. A standard 50 ml ultrafiltration (UF) cell was used as semicontinuous reactor. The solution containing cyclopropanol treated cell suspension and sodium formate in phosphate buffer was introduced in to the UF cell attached with ultrafilter (Diaflo ultrafilter YM-100, Amicon, Inc.). The UF cell was incubated for 5 min at 30ﾟC,and the reaction was initiated by injecting methane into the UF cell with a gas-tight syringe. After incubation at 30ﾟC for 90 min, the reaction mixture was filtrated by nitrogen pressure, leading to separate a produced methanol from cell suspension. The methanol synthesis was repeated five times and stationary rate was 3.17 mumol h^<-1>mg^<-1>. The total produced methanol was 36.1 mumol, which is about twice amount of methanol produced by batch reaction under the same condition. Less

与由通过蒸汽将甲烷转化成合成气的能量密集型转化组成的现有方法相比，甲烷直接氧化成甲醇是非常有吸引力的方法。甲烷单加氧酶（MMO）催化甲烷一步氧化为甲醇。但由于MMO的不稳定性，不能用于甲醇合成。因此，用甲烷氧化菌尝试从甲烷生产甲醇。Methylosinus trichosporium OB 3b是一株含MMO的甲烷氧化菌，催化甲烷羟基化生成甲醇，当甲烷被氧化时，所生成的甲醇又被该菌所含的甲醇脱氢酶氧化。为了防止甲醇的进一步氧化，细胞悬浮液用环丙醇处理，环丙醇被发现是甲醇脱氢酶的不可逆抑制剂，导致细胞外甲醇积累。本研究的目的是增加甲醇产量，提高甲醇收率， ...更多信息通过发孢霉OB 3b的分批型甲醇合成在约6 mM甲醇时终止，因为MMO通过增加甲醇浓度而被抑制。为了延长甲醇积累，在本研究中进行了一个连续的过程。使用标准的50 ml超滤（UF）池作为连续反应器。将含有环丙醇处理的细胞悬浮液和甲酸钠的磷酸盐缓冲液溶液引入连接有超滤器（Diaflo超滤器YM-100，Amicon，Inc.）的UF池中。将UF池在30 ° C下孵育5分钟，并通过用气密注射器将甲烷注入UF池中来引发反应。在30 ° C温育90分钟后，通过氮气压力过滤反应混合物，从而从细胞悬浮液中分离产生的甲醇。甲醇合成重复5次，固定速率为3.17 μ mol·h·<-1>mg·L-1<-1>。总甲醇产量为36.1 μ mol，约为相同条件下间歇反应甲醇产量的2倍。少