Directed Evolution of Monooxygenases for Green Chemistry, Methanol Production, and Rhizoremediation

用于绿色化学、甲醇生产和根修复的单加氧酶的定向进化

• 批准号: 9807146
• 负责人: Thomas Wood
• 金额: $ 22.36万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-15 至 2001-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9807146&HistoricalAwards=false
• 关键词: Directed; Evolution; Monooxygenases; Green; Chemistry

This project is to explore the use of a family of monooxygenases for the production of chiral chemicals without using harsh organics, for the production of methanol, and for the remediation of chlorinated aliphatics using a novel rhizoremediation process. An optimized family of monooxygenases is to be created using a DNA shuffling technique. These monooxygenases are to be specifically designed for the degradation of trichloroethylene, the formation of methanol, and for the synthesis of chiral compounds such as styrene epoxide and alpha-naphthol. An innovative and powerful selection technique is to be used to isolate the engineered proteins.

该项目是探索使用一个家庭的单加氧酶生产手性化学品，而不使用苛刻的有机物，生产甲醇，并使用一种新的rhizoremediation过程中的氯农药的补救。 使用DNA改组技术创建单加氧酶的优化家族。 这些单加氧酶是专门设计用于三氯乙烯的降解，甲醇的形成，并用于合成手性化合物，如环氧苯乙烯和α-萘酚。 一个创新的和强大的选择技术是用来分离工程蛋白质。