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Directed Evolution of Monooxygenases for Green Chemistry, Methanol Production, and Rhizoremediation

Directed Evolution of Monooxygenases for Green Chemistry, Methanol Production, and Rhizoremediation
用于绿色化学、甲醇生产和根修复的单加氧酶的定向进化
批准号:
9807146
负责人:
Thomas Wood
金额:
$22.36万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-12-15 至 2001-11-30

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中文摘要
翻译
本项目旨在探索利用单加氧酶家族在不使用有害有机物的情况下生产手性化学品,生产甲醇,以及利用一种新的根茎修复工艺修复氯化脂肪族。一个优化家族的单加氧酶是创建使用DNA洗牌技术。这些单加氧酶是专门设计用于降解三氯乙烯,形成甲醇,以及合成手性化合物,如环氧苯乙烯和-萘酚。一个创新的和强大的选择技术是用来分离工程蛋白。
英文摘要
This project is to explore the use of a family of monooxygenases for the production of chiral chemicals without using harsh organics, for the production of methanol, and for the remediation of chlorinated aliphatics using a novel rhizoremediation process. An optimized family of monooxygenases is to be created using a DNA shuffling technique. These monooxygenases are to be specifically designed for the degradation of trichloroethylene, the formation of methanol, and for the synthesis of chiral compounds such as styrene epoxide and alpha-naphthol. An innovative and powerful selection technique is to be used to isolate the engineered proteins.
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