Treamtent of liver cirrhosis using inoculation of proliferative hepatocyte and gene transduction of antisense for mRNA of collagenase inhibitor.

实验一：用四氯化碳和二甲基亚硝胺(DMNA)分别对小鼠和大鼠建立肝硬变动物模型。实验二：为了制作具有肝细胞功能和增殖功能的杂交瘤，我们尝试用激光制作细胞融合装置。实验三：为了通过基因转导来治疗细胞功能，我们进行了反义肿瘤坏死因子基因的脂质体包裹，并将其导入Kupffer细胞的初步研究。反义治疗后，肿瘤坏死因子的产量下降到40%。

Experiment 1 : For establishment of a animal model with liver cirrhosis, CCl_4 and dimethyl nitrosoamine (DMNA) were administered to mouse and rat. Liver of animals with administration of CCl_4 were cirrhotic but parenchymal hepatocyte was injured so severely.Experiment 2 : For making hybridoma which has function of hepatocyte and proliferates, we tried to make cell fusion equipment using laser. Using this device, we could make hybridoma from mouse myeloma cells and lymphocytes.Experiment 3 : For treatment of cell function by gene transduction, we carried out the preliminary study that antisense for mRNA of TNF was encapsuled with HVJ liposome and was transfected to Kupffer cells. With antisense therapy, production of TNF decreased to 40%.