Treamtent of liver cirrhosis using inoculation of proliferative hepatocyte and gene transduction of antisense for mRNA of collagenase inhibitor.

实验一：用四氯化碳（CCl_4）和二甲基亚硝胺（DMNA）分别给小鼠和大鼠建立肝硬化动物模型。实验二：为了制备具有肝细胞功能并能增殖的杂交瘤细胞，我们尝试用激光制作细胞融合装置。实验3：为探讨基因转导对细胞功能的影响，我们进行了用HVJ脂质体包裹TNF反义mRNA转染Kupffer细胞的初步研究。用反义治疗，TNF的产生减少到40%。

Experiment 1 : For establishment of a animal model with liver cirrhosis, CCl_4 and dimethyl nitrosoamine (DMNA) were administered to mouse and rat. Liver of animals with administration of CCl_4 were cirrhotic but parenchymal hepatocyte was injured so severely.Experiment 2 : For making hybridoma which has function of hepatocyte and proliferates, we tried to make cell fusion equipment using laser. Using this device, we could make hybridoma from mouse myeloma cells and lymphocytes.Experiment 3 : For treatment of cell function by gene transduction, we carried out the preliminary study that antisense for mRNA of TNF was encapsuled with HVJ liposome and was transfected to Kupffer cells. With antisense therapy, production of TNF decreased to 40%.