The study of invasion and proliferation status in oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和增殖状态的研究
基本信息
- 批准号:08457544
- 负责人:
- 金额:$ 4.29万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1996
- 资助国家:日本
- 起止时间:1996 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The vascular architecture of DMBA (9, 10-dimethyl I, 2-benzanthracene) induced tongue carcinoma in the hamster was examined. Changes in tumor vessels and their significance during growth of invasive oral squamous cell carcinoma was observed. Tongue cancer was induced by abrading the right margin of the tongue of each hamster with an endodontic barbed broach and subsequently applying 1.0 % DMBA dissolved in aceton, three times a week, at the same site. After macroscopic detection of cancer and the signs of cachexia (weight loss, etc.), Indian-ink was infused into the ascending aorta of each animal under general anesthesia. Tissue was harvested from each animal and made into a transparent specimen, 100 p m in thickness, for examination of vasucular architecture. The mode of cancer invasion was Grade I in 8 animals, Grade 2 in 8, Grade 3 in 23, and Crade 4C in 5. Macroscopic tumor growth mode was exophytic in 36 animals and endophytic in 8 animals. Each mode of cancer invasion was associa … More ted with characteristic vascular architecture. That is, low invasive cancers (Grade I and 2) showed dendriform vascularization and a marked increase in vessel density as they underwent exophitic growth. Grade 3 invasive cancei shbwed rings of blood vessels forming during the coume of invasion by alveolar tumor foci. Diffusely invasive cancer (Grade 4C) showed destruction of exisdng blood vessels and a decrease in vessel density. As fumor invasion progressed ; the vessel density decreased (p<0.05). The vessel density was significantly lower in cases of exophytic cancer than in cases of endophytic cancer (p<0.01). The PCNA (proliferating cell nuclear antigen) of cancer cells, which is an indicator of tumor proliferation potential, was more frequently positive as tumor invasion bccame more severe. That is, the PCNA positive rate in Grade I or 2 animals differed significantly from that in Grade 3 or 4C animals (p<0.05). The PCNA positive rate decreased as vessel density increased (p<0.05). Thus, the vessel density was not proportional to the tumor proliferation potential. The expression of vascular endothelial growth factor (VEGF) was seen more frequently as tumor invasion progressed (p<0.05), but the expression of VEGF did not correlate with the vessel density. These results suggested that the changes of the tumor vessel were dependent on the mode of cancer invasion and proliferation. Less
本文观察了DMBA(9,10-二甲基-1,2-苯并蒽)诱发的金黄地鼠舌癌的血管构筑。本文观察了口腔浸润性鳞癌生长过程中肿瘤血管的变化及其意义。通过用牙髓倒钩拉刀刮擦每只仓鼠舌头的右边缘,随后在同一部位施用溶解在丙酮中的1.0%DMBA,每周三次,诱导舌癌。在肉眼检测到癌症和恶病质迹象(体重减轻等)后,在全身麻醉下将印度墨水注入每只动物的升主动脉。从每只动物中收获组织并制成厚度为100 μ m的透明标本,用于检查血管结构。8只动物的癌症侵袭模式为I级,8只为2级,23只为3级,5只为Crade 4C。肉眼可见肿瘤生长方式为外生性36只,内生性8只。每种癌症侵袭模式都是相关的 ...更多信息 具有典型的血管结构。也就是说,低侵袭性癌症(I级和2级)表现出树状血管形成和血管密度的显着增加,因为它们经历了外突生长。3级浸润性癌在肺泡肿瘤病灶浸润过程中出现血管环。弥漫性浸润癌(4C级)显示血管破坏和血管密度降低。随着肿瘤浸润的进展,血管密度逐渐降低(P<0.05)。外生型肿瘤的血管密度明显低于内生型肿瘤(P<0.01)。癌细胞的增殖细胞核抗原(PCNA)是肿瘤增殖能力的一个指标,随着肿瘤浸润程度的增加,PCNA阳性率也越来越高。也就是说,I级或2级动物的PCNA阳性率与3级或4C级动物的PCNA阳性率存在显著差异(p<0.05)。PCNA阳性率随血管密度的增加而降低(p<0.05)。因此,血管密度与肿瘤增殖潜力不成比例。血管内皮生长因子(VEGF)的表达随肿瘤浸润深度的增加而增加(p<0.05),但VEGF的表达与肿瘤血管密度无关。提示肿瘤血管的变化与肿瘤的侵袭和增殖方式有关。少
项目成果
期刊论文数量(19)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Shuichi Kawashiri: "Occult metastasis of cancer cells in an orthotopic implanted oral cancer model-Reproduce the occult metastasis.(Proceeding of the 5th International Congress on Oral Cancer=ICOOC,LOndon,UK): Editor;Varma,AK,Macmillan India Limited" Oral
Shuichi Kawashiri:“原位种植口腔癌模型中癌细胞的隐匿性转移 - 再现隐匿性转移。(第五届国际口腔癌大会论文集=ICOOC,伦敦,英国):编辑;Varma,AK,Macmillan India Limited”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Moriyama, M.: "Tumor angiogenesis and expression of vascular endotherial growth factor in oral squamous cell carcinoma." Head and Neck Cancer. 24(1). 12-17 (1998)
Moriyama, M.:“口腔鳞状细胞癌中的肿瘤血管生成和血管内皮生长因子的表达。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kakihara,K: "A study of the angiogenesis of oral squamous cell carcinoma.(Proceeding of the 5th International Congress on Oral Cancer=ICOOC,LOndon,UK): Editor; Varma,AK,Macmillan India Limited" Oral Oncol. 5. 431-434 (1997)
Kakihara,K:“口腔鳞状细胞癌血管生成的研究。(第五届国际口腔癌大会论文集=ICOOC,伦敦,英国):编辑;Varma,AK,Macmillan India Limited”Oral Oncol。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
熊谷茂宏: "ヒトロ腔扁平上皮癌組織における膜型マトリックスメタロプロテアーゼの発現" 日本口腔腫瘍学会誌. 10(1). 9-15 (1998)
Shigehiro Kumagai:“人腔鳞状细胞癌组织中膜型基质金属蛋白酶的表达”日本口腔肿瘤学会杂志10(1)(1998)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kumagai, S.: "Expression of membrane type-matrix metalloproteinases in human oral squamous cell carcinomas." J Jpn Soc Oral Tumor. 10(1). 9-15 (1998)
Kumagai, S.:“膜型基质金属蛋白酶在人类口腔鳞状细胞癌中的表达。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
YAMAMOTO Etsuhide其他文献
YAMAMOTO Etsuhide的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('YAMAMOTO Etsuhide', 18)}}的其他基金
Investigation the mechanisms on invasion and metastasisof oral squamous cell carcinoma
口腔鳞状细胞癌侵袭转移机制探讨
- 批准号:
22390382 - 财政年份:2010
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Experimental stucles of invasion and metastasis adivky of oral squamous cdl carcinoma
口腔鳞状细胞癌侵袭转移实验研究
- 批准号:
17390533 - 财政年份:2005
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Experimental studies of invasion and metastasis activity of oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和转移活性的实验研究
- 批准号:
14370666 - 财政年份:2002
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Experimental studies of invasive and proliferative activity of oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和增殖活性的实验研究
- 批准号:
11470432 - 财政年份:1999
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies of invasive and proliferative activity of oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和增殖活性的研究
- 批准号:
06454567 - 财政年份:1994
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)