The study of invasion and proliferation status in oral squamous cell carcinoma

研究了DMBA（9,10 -二甲基I， 2-苯并蒽）诱导的鼠舌癌的血管结构。观察侵袭性口腔鳞状细胞癌生长过程中肿瘤血管的变化及其意义。舌癌的诱发方法是：用带牙髓的有刺的拉针在每只仓鼠的舌右缘上刮擦，随后在同一部位涂抹1.0%的丙酮溶解DMBA，每周三次。肉眼检查出癌变及恶病质体征（体重减轻等）后，在全身麻醉下将墨汁注入每只动物的升主动脉。从每只动物身上采集组织，制成厚度为100微米的透明标本，用于检查血管结构。肿瘤侵袭模式为I级8只，2级8只，3级23只，4C级5只。肉眼可见肿瘤生长方式为外生36只，内生8只。每一种癌症侵袭模式都与特征性的血管结构有关。也就是说，低侵袭性肿瘤（I级和2级）在发生外生性生长时表现为树突状血管形成，血管密度显著增加。3级浸润性癌表现为肺泡肿瘤灶浸润时形成的血管环。弥漫性浸润癌（4C级）表现为现有血管的破坏和血管密度的降低。随着肿瘤侵袭的进展；血管密度降低（p<0.05）。外生性肿瘤的血管密度显著低于内生肿瘤（p<0.01）。肿瘤细胞的增殖细胞核抗原（PCNA）是肿瘤增殖潜能的一个指标，随着肿瘤侵袭程度的加重，PCNA阳性的频率更高。即1级、2级动物的PCNA阳性率与3级、4级动物的PCNA阳性率差异有统计学意义（p<0.05）。PCNA阳性率随血管密度的增加而降低（p<0.05）。因此，血管密度与肿瘤增殖潜能不成正比。随着肿瘤侵袭的进展，血管内皮生长因子（VEGF）的表达增多（p<0.05），但VEGF的表达与血管密度无关。这些结果提示肿瘤血管的改变依赖于肿瘤侵袭和增殖的方式。少

The vascular architecture of DMBA (9, 10-dimethyl I, 2-benzanthracene) induced tongue carcinoma in the hamster was examined. Changes in tumor vessels and their significance during growth of invasive oral squamous cell carcinoma was observed. Tongue cancer was induced by abrading the right margin of the tongue of each hamster with an endodontic barbed broach and subsequently applying 1.0 % DMBA dissolved in aceton, three times a week, at the same site. After macroscopic detection of cancer and the signs of cachexia (weight loss, etc.), Indian-ink was infused into the ascending aorta of each animal under general anesthesia. Tissue was harvested from each animal and made into a transparent specimen, 100 p m in thickness, for examination of vasucular architecture. The mode of cancer invasion was Grade I in 8 animals, Grade 2 in 8, Grade 3 in 23, and Crade 4C in 5. Macroscopic tumor growth mode was exophytic in 36 animals and endophytic in 8 animals. Each mode of cancer invasion was associa … More ted with characteristic vascular architecture. That is, low invasive cancers (Grade I and 2) showed dendriform vascularization and a marked increase in vessel density as they underwent exophitic growth. Grade 3 invasive cancei shbwed rings of blood vessels forming during the coume of invasion by alveolar tumor foci. Diffusely invasive cancer (Grade 4C) showed destruction of exisdng blood vessels and a decrease in vessel density. As fumor invasion progressed ; the vessel density decreased (p<0.05). The vessel density was significantly lower in cases of exophytic cancer than in cases of endophytic cancer (p<0.01). The PCNA (proliferating cell nuclear antigen) of cancer cells, which is an indicator of tumor proliferation potential, was more frequently positive as tumor invasion bccame more severe. That is, the PCNA positive rate in Grade I or 2 animals differed significantly from that in Grade 3 or 4C animals (p<0.05). The PCNA positive rate decreased as vessel density increased (p<0.05). Thus, the vessel density was not proportional to the tumor proliferation potential. The expression of vascular endothelial growth factor (VEGF) was seen more frequently as tumor invasion progressed (p<0.05), but the expression of VEGF did not correlate with the vessel density. These results suggested that the changes of the tumor vessel were dependent on the mode of cancer invasion and proliferation. Less