Studies of invasive and proliferative activity of oral squamous cell carcinoma

口腔鳞状细胞癌侵袭和增殖活性的研究

• 批准号: 06454567
• 负责人: YAMAMOTO Etsuhide
• 金额: $ 0.96万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454567/
• 关键词: Orthotopic implantaion; Invasion and metastasis; Oral squamous cell carcinoma; Type IV collagen; Desmoglein; Proliferating cell nuclear antigen; Matrix metalloproteinase; In vivo浸潤・転移モデル; 浸潤様式; IV型コラーゲン; デスモグレインI; 増殖細胞核抗原(PCNA)

We devised a new model in order to establish an in vivo model for human oral carcinoma that exhibits significant local invasion and metastasis. In the present study, we examined the invasive sctivity of five kinds of oral squamous carcinoma cells (OSC-19, OSC-20, SS, FA and TT) by this new model. The nude mice had tumor cells from one of five oral squamous cell carcinoma cells implanted into the tongue or the oral floor via an intra-oral route and, as a control, the subcutaneous tissue of the back. The back tumors showed a benign growth pattern, lacking significant invasion of surrounding tissues. In contrast, the tumors implanted into the tongue or the oral floor exhibited invasive growth and stromal reactions, the histological appearance of which was similar to that of the original tumors. Moreover, the regional neck lymph node and pulmonary metastases were observed in this model. Regional neck lymph node metastases were detected in 81.0% of mice implanted with OSC-19 cells. The oral tumor implanted with OSC-19 cells or TT cells showed a cords-like diffuse invasion pattern and was characterized by the abscence of a continuous basement membrane and by a reduction in the intercellular adhesion of carcinoma cells. These results suggest that the invasion of oral squamous cell carcinoma may be influenced by the tumor-stromal and/or intercellular interactions.

我们设计了一种新的模型，以建立一个在体内模型的人口腔癌，表现出显着的局部浸润和转移。本研究应用该模型检测了五种口腔鳞癌细胞（OSC-19、OSC-20、SS、FA和TT）的侵袭能力。裸鼠的肿瘤细胞来自五个口腔鳞状细胞癌细胞之一，通过口内途径植入舌头或口底，作为对照，背部的皮下组织。背部肿瘤呈良性生长，无明显侵犯周围组织。相反，植入舌或口底的肿瘤表现出侵袭性生长和间质反应，其组织学外观与原始肿瘤相似。此外，在该模型中还观察到区域性颈淋巴结和肺转移。在植入OSC-19细胞的小鼠中检测到81.0%的区域颈淋巴结转移。移植OSC-19细胞或TT细胞的口腔肿瘤表现为索状弥漫浸润模式，其特征在于连续基底膜的破坏和癌细胞间粘附的减少。这些结果表明，口腔鳞状细胞癌的侵袭可能受到肿瘤间质和/或细胞间相互作用的影响。

项目成果

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Yamamoto E.等人：“从侵袭模式的角度来看口腔癌术前化疗的作用。”

Kawashiri, S., kumagai, S., Kojima, K., Harada, H., Yamamoto, E.: "Development of a new invasion and metastasis model of human oral squamous cell c arcinomas." Oral Oncol, Eur J Cancer. 31B. 216-221 (1995)

Kawashiri, S.、kumagai, S.、Kojima, K.、Harada, H.、Yamamoto, E.：“开发人类口腔鳞状细胞癌的新侵袭和转移模型。”

Yamamoto E.et al.: "Development of a new invasion and metastasis model of human oral squamous cell carcinomas." Oral Oncology,European Journal of Cancer. 31B. 216-221 (1995)

Yamamoto E.等人：“开发一种新的人类口腔鳞状细胞癌侵袭和转移模型。”

Kumagai, S., Imai, K., Nakagawa, K., Yamamoto, E., Nakanishi, I., Okada., Y.: "Immunohistologic distribution of basement membrane in oral squamous cell carcinoma." Head & Neck. 16. 58-63 (1994)

Kumagai, S.、Imai, K.、Nakakawa, K.、Yamamoto, E.、Nakanishi, I.、Okada., Y.：“口腔鳞状细胞癌中基底膜的免疫组织学分布。”

Imai, K., Kumagai, S.Nakagawa, K., Yamamoto, E., Nakanishi, I., Okada, Y.: "Immuno-localization of desmoglein and intermediate filaments in human oral squamous cell carcinomas." Head & Neck. 17. 204-212 (1995)

Imai, K.、Kumagai、S.Nakakawa, K.、Yamamoto, E.、Nakanishi, I.、Okada, Y.：“人类口腔鳞状细胞癌中桥粒芯糖蛋白和中间丝的免疫定位。”