Studies of invasive and proliferative activity of oral squamous cell carcinoma

我们设计了一种新的口腔癌模型，以建立具有明显局部侵袭和转移的人口腔癌的体内模型。在本研究中，我们用这种新模型检测了五种口腔鳞癌细胞（osc19、osc20、SS、FA和TT）的侵袭性。裸鼠将五种口腔鳞状细胞癌细胞中的一种的肿瘤细胞通过口腔内途径植入舌头或口腔底，作为对照，植入背部皮下组织。背部肿瘤呈良性生长模式，对周围组织无明显侵犯。而植入舌部或口底的肿瘤则表现为侵袭性生长和间质反应，其组织学表现与原肿瘤相似。此外，该模型还观察到局部颈部淋巴结和肺转移。81.0%的小鼠移植oc -19细胞后出现颈部局部淋巴结转移。移植OSC-19细胞或TT细胞的口腔肿瘤呈索状弥漫性浸润，基底膜脱落，细胞间黏附减少。这些结果提示口腔鳞状细胞癌的侵袭可能受到肿瘤-基质和/或细胞间相互作用的影响。

We devised a new model in order to establish an in vivo model for human oral carcinoma that exhibits significant local invasion and metastasis. In the present study, we examined the invasive sctivity of five kinds of oral squamous carcinoma cells (OSC-19, OSC-20, SS, FA and TT) by this new model. The nude mice had tumor cells from one of five oral squamous cell carcinoma cells implanted into the tongue or the oral floor via an intra-oral route and, as a control, the subcutaneous tissue of the back. The back tumors showed a benign growth pattern, lacking significant invasion of surrounding tissues. In contrast, the tumors implanted into the tongue or the oral floor exhibited invasive growth and stromal reactions, the histological appearance of which was similar to that of the original tumors. Moreover, the regional neck lymph node and pulmonary metastases were observed in this model. Regional neck lymph node metastases were detected in 81.0% of mice implanted with OSC-19 cells. The oral tumor implanted with OSC-19 cells or TT cells showed a cords-like diffuse invasion pattern and was characterized by the abscence of a continuous basement membrane and by a reduction in the intercellular adhesion of carcinoma cells. These results suggest that the invasion of oral squamous cell carcinoma may be influenced by the tumor-stromal and/or intercellular interactions.