Studies of invasive and proliferative activity of oral squamous cell carcinoma

我们设计了一种新的模型，以建立一个在体内模型的人口腔癌，表现出显着的局部浸润和转移。本研究应用该模型检测了五种口腔鳞癌细胞（OSC-19、OSC-20、SS、FA和TT）的侵袭能力。裸鼠的肿瘤细胞来自五个口腔鳞状细胞癌细胞之一，通过口腔内途径植入舌头或口底，作为对照，背部的皮下组织。背部肿瘤呈良性生长，无明显侵犯周围组织。相反，植入舌或口底的肿瘤表现出侵袭性生长和间质反应，其组织学外观与原始肿瘤相似。此外，在该模型中还观察到区域性颈淋巴结和肺转移。在植入OSC-19细胞的小鼠中检测到81.0%的区域颈淋巴结转移。移植OSC-19细胞或TT细胞的口腔肿瘤表现为索状弥漫浸润模式，其特征在于连续基底膜的破坏和癌细胞间粘附的减少。这些结果表明，口腔鳞状细胞癌的侵袭可能受到肿瘤间质和/或细胞间相互作用的影响。

We devised a new model in order to establish an in vivo model for human oral carcinoma that exhibits significant local invasion and metastasis. In the present study, we examined the invasive sctivity of five kinds of oral squamous carcinoma cells (OSC-19, OSC-20, SS, FA and TT) by this new model. The nude mice had tumor cells from one of five oral squamous cell carcinoma cells implanted into the tongue or the oral floor via an intra-oral route and, as a control, the subcutaneous tissue of the back. The back tumors showed a benign growth pattern, lacking significant invasion of surrounding tissues. In contrast, the tumors implanted into the tongue or the oral floor exhibited invasive growth and stromal reactions, the histological appearance of which was similar to that of the original tumors. Moreover, the regional neck lymph node and pulmonary metastases were observed in this model. Regional neck lymph node metastases were detected in 81.0% of mice implanted with OSC-19 cells. The oral tumor implanted with OSC-19 cells or TT cells showed a cords-like diffuse invasion pattern and was characterized by the abscence of a continuous basement membrane and by a reduction in the intercellular adhesion of carcinoma cells. These results suggest that the invasion of oral squamous cell carcinoma may be influenced by the tumor-stromal and/or intercellular interactions.