Experimental studies of invasion and metastasis activity of oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和转移活性的实验研究
基本信息
- 批准号:14370666
- 负责人:
- 金额:$ 8.9万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Since 1978, we have been studying the mode of cancer invasion as the important prognostic factor in patients with oral squamous cell cancer. Grade 4 of mode of invasion proposed by Jakobsson was subclassified into grades 4C (Cord-like type) and 4D (Diffuse type). The purpose of this study is to immunohistochemically and experimentally elucidate this modified grading system for the mode of invasion. Materials and methods used are human cancer materials, DMBA induced tongue cancer and in vitro and in vivo invasion model by using 3 cell lines ; OCS-20 (Gr.3), OSC-19 (Gr.4C) and HOC-313 (Gr.4D). In immunostained human cancer materials, the higher the grade of the mode of invasion was, the less continuous the basement membrane was, and the less intercellular adhesion was. Majority of diffuse invasive cancers(Gr.4C and 4D) co-expressed membrane type-matrix metalloproteinase and matrix metalloprotenase-2. In cell motility assay, HOC-313 showed the greatest motility (autocrine motility factor ; AMF). Cell lines cultured on 3T3 fibroblast-embedded collagen gels showed similar invasion pattern to their origin. Among them, only HOC-313 showed invasion even if fibroblast was not embedded. In DMBA induced tongue cancer, the mode of invasion were observed from Gr.1 to Gr.4C. OSC-20 and OSC-19 implanted orthotopically in the tongue of nude mice grew and metastasized to lymph nodes. Their histologic appearance was similar to that of the origin. HOC-313 grew temporarily for several months by support of matrigel. In recent study, cell selected TSU (Gr.4D) produced lethal tumorgenicity in SCID mouse with similar histology to the origin. This grading system was also proven immunohistochemically and experimentally justifiable.
自1978年以来,我们一直在研究口腔鳞状细胞癌患者的重要预后因素癌症的侵袭方式。Jakobsson提出的4级侵犯模式又分为4C级(索状型)和4D级(弥漫型)。本研究的目的是化学和实验阐明这种修改后的分级系统的入侵模式。使用的材料和方法是人癌材料、DMBA诱导的舌癌和使用3种细胞系的体外和体内侵袭模型; OCS-20(Gr.3)、OSC-19(Gr.4C)和HOC-313(Gr.4D)。在免疫组化染色的人癌组织中,浸润方式分级越高,基底膜越不连续,细胞间粘附越少。大多数弥漫性浸润癌(Gr.4C和4D)共表达膜型基质金属蛋白酶和基质金属蛋白酶-2。在细胞运动性测定中,HOC-313显示最大的运动性(自分泌运动因子; AMF)。在3 T3成纤维细胞包埋的胶原凝胶上培养的细胞系显示出与其来源相似的侵袭模式。其中,即使成纤维细胞没有包埋,只有HOC-313表现出侵袭性。DMBA诱发的舌癌细胞的侵袭方式为Gr.1 ~ Gr.4C。原位植入裸鼠舌中的OSC-20和OSC-19生长并转移至淋巴结。它们的组织学外观与起源相似。HOC-313在基质胶的支持下暂时生长了几个月。最近的研究表明,细胞选择性大津单位(Gr.4D)在SCID小鼠中产生了致死性致瘤性,其组织学与起源相似。这种分级系统也被证明是化学和实验合理的。
项目成果
期刊论文数量(20)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Expression of the active type gelatinase and MMPs of oral squamous cell carcinoma.
口腔鳞状细胞癌活性型明胶酶和基质金属蛋白酶的表达。
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Kawashiri S.;Tanaka A.;Yamamoto E.;et al.
- 通讯作者:et al.
Efficacy of neoadjuvant chemotherapy on metastasis of oral squamous cell carcinoma in mouse model
新辅助化疗对小鼠口腔鳞状细胞癌转移模型的影响
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Kawashiri S.;Tanaka A.;Noguchi N.;et al.
- 通讯作者:et al.
Noguchi N., Kawashiri S., 他: "Effects of fibroblast growth inhibitor on proliferation and metastasis Of oral squamous cell carcinoma"Oral Oncology. 39・3. 240-247 (2003)
Noguchi N.、Kawashiri S.等人:“成纤维细胞生长抑制剂对口腔鳞状细胞癌增殖和转移的影响”口腔肿瘤学39·3(2003)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Effects of fibroblast groeth inhibitor on proliferation and metastasis of oral squamous cell carcinoma
成纤维细胞生长抑制剂对口腔鳞癌增殖和转移的影响
- DOI:
- 发表时间:2002
- 期刊:
- 影响因子:0
- 作者:Noguchi N.;Kawashiri S.;Tanaka A.;et al.
- 通讯作者:et al.
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YAMAMOTO Etsuhide其他文献
YAMAMOTO Etsuhide的其他文献
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{{ truncateString('YAMAMOTO Etsuhide', 18)}}的其他基金
Investigation the mechanisms on invasion and metastasisof oral squamous cell carcinoma
口腔鳞状细胞癌侵袭转移机制探讨
- 批准号:
22390382 - 财政年份:2010
- 资助金额:
$ 8.9万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Experimental stucles of invasion and metastasis adivky of oral squamous cdl carcinoma
口腔鳞状细胞癌侵袭转移实验研究
- 批准号:
17390533 - 财政年份:2005
- 资助金额:
$ 8.9万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Experimental studies of invasive and proliferative activity of oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和增殖活性的实验研究
- 批准号:
11470432 - 财政年份:1999
- 资助金额:
$ 8.9万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The study of invasion and proliferation status in oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和增殖状态的研究
- 批准号:
08457544 - 财政年份:1996
- 资助金额:
$ 8.9万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies of invasive and proliferative activity of oral squamous cell carcinoma
口腔鳞状细胞癌侵袭和增殖活性的研究
- 批准号:
06454567 - 财政年份:1994
- 资助金额:
$ 8.9万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
相似海外基金
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PD-L1/PD-1信号参与口腔鳞癌的侵袭和转移
- 批准号:
17K17264 - 财政年份:2017
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口腔鳞癌侵袭转移机制的阐明及侵袭转移的预防
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15H05042 - 财政年份:2015
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$ 8.9万 - 项目类别:
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Approach to the mechanism of invasion and metastasis in oral squamous cell carcinoma from sphingolipid.
鞘脂对口腔鳞状细胞癌侵袭和转移机制的探讨。
- 批准号:
26670875 - 财政年份:2014
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26463038 - 财政年份:2014
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- 批准号:
26670861 - 财政年份:2014
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$ 8.9万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Role of cancer stem cells and epithelial-mesenchymal transition in the invasion and metastasis in oral squamous cell carcinoma
肿瘤干细胞和上皮间质转化在口腔鳞癌侵袭和转移中的作用
- 批准号:
25462940 - 财政年份:2013
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Role of the adhesion molecules between the cell in invasion, the metastasis of oral squamous cell carcinoma
细胞间粘附分子在口腔鳞癌侵袭、转移中的作用
- 批准号:
25861923 - 财政年份:2013
- 资助金额:
$ 8.9万 - 项目类别:
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Epithelial mesenchymal transition induced invasion and metastasis in oral squamous cell carcinoma via delta-Np63.
上皮间质转化通过 delta-Np63 诱导口腔鳞状细胞癌的侵袭和转移。
- 批准号:
25870492 - 财政年份:2013
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$ 8.9万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Effects of angiogenesis inhibitor and fibroblast growth inhibitor on invasion and metastasis of oral squamous cell carcinoma.
血管生成抑制剂和成纤维细胞生长抑制剂对口腔鳞癌侵袭和转移的影响。
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24792195 - 财政年份:2012
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