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Molecular biological relationships between oral cavity and upper gastrointestinal tract in Helicobacter pylori infection

幽门螺杆菌感染中口腔与上消化道的分子生物学关系

基本信息

批准号：
08457546
负责人：
IIZUKA Tadahiko
金额：
$ 5.57万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457546/
关键词：
Helicobacter Pylori eradication therapy dental plaque saliva IgA oral immunization oral cancer MALT lymphoma ヘリコバクター・ピロリ口腔疾患マウス IgA抗体 IgG抗体口腔粘膜疾患 PCR ポリクロナール抗体アポトーシス TUNEL

项目摘要

To analyze the existence of Helicobacter pylori (H. pylori) in the oral cavity, we collected the plaque and saliva in the patients with oral disease, and tested the existence of H. pylori, using PCR technique. As a result, H. pylori existed in the oral cavity in proportion to ages, especially which was detected in 50% among over 40 ages.Next, to know the influence of oral H. pylori on the gastric reinfection and the correlation between the prevalence of H. pylori in the oral cavity and the gastric eradication success, we tested the dental plaque control in infected patients, when they were treated with chemotherapy. As a result, three of four patients are detected with H. pylori in the oral cavity before and after eradication therapy, but H. pylori were not detected in the oral cavity after dental plaque control. In the stomach, after the treatment of eradication therapy followed by dental plaque control, all the patients were negative for CLO test in 3, 6 and 12 months. Our result sug … More gests that H. pylori in the oral cavity affect the outcome of eradication therapy and was associated with a recurrence of gastric infection. We recommend that oral H. pylori should be examined by PCR before eradication therapy, and if positive, should be treated with dental plaque control.Thirdly, we studied the role of saliva in the induction and maintenance of the immunity in mice conferred by oral vaccination against H. pylori. As a result, oral vaccination in the mice who underwent a sialoadenctomy before oral immunization was no less effective in the mice who underwent a sialoadenectomy after the completion of immunization at 1 month, and the efficacy of immunization was significantly reduced by sialoadenectomy at 6 months after immunization. We found that saliva is necessary about the protection from the H. pylori infection and immune induction and maintenance.To examine the correlation between the prevalence of H. pylori in the oral cavity and oral cancer, we examined immunohistochemistry. 40 patients who were referred to Kyoto University Hospital between 1994 and 1998 with oral tumor were enrolled in this study. As a result, we assessed the existence of H. pylori in 3 of 40 patients. We also examined immunohisochemistry in MALT lymphoma arising in the oral cavity. We assessed the existence of H. pylori in the salivary duct. However, further investigation is required to know about the pathogenic mechanism. Less

分析幽门螺杆菌（H. pylori）的感染情况，收集口腔疾病患者的菌斑和唾液，检测H. pylori感染。因此，H. pylori在口腔中的检出率随年龄的增长而增加，尤其是40岁以上的人群中检出率高达50%。pylori对胃内再感染的影响与H.幽门螺杆菌在口腔和胃根除成功后，我们测试了感染患者的牙菌斑控制，当他们接受化疗治疗。结果，四分之三的患者被检测出H。幽门螺杆菌根除治疗前后口腔内的幽门螺杆菌均为阴性，而幽门螺杆菌根除治疗前后口腔内的幽门螺杆菌均为阴性。pylori在控制牙菌斑后的口腔中未检测到。在胃，根除治疗后，牙菌斑控制治疗，所有患者在3，6和12个月的CLO试验阴性。我们的结果表明， ...更多信息他认为H。口腔中的幽门螺杆菌影响根除治疗的结果，并与胃感染的复发有关。我们建议口服H。pylori感染的小鼠，在进行根除治疗前应进行PCR检测，如阳性，应进行菌斑控制治疗。幽门螺杆菌。结果，在口服免疫前进行了涎腺切除术的小鼠中的口服疫苗接种在完成免疫后1个月进行了涎腺切除术的小鼠中同样有效，并且在免疫后6个月进行了涎腺切除术的小鼠中免疫效力显著降低。我们发现，唾液是必要的保护，从H。pylori感染与免疫诱导和维持的关系。pylori在口腔和口腔癌中的表达，我们进行了免疫组化检查。本研究纳入了1994年至1998年期间转诊至京都大学医院的40例口腔肿瘤患者。因此，我们评估了H的存在。40例患者中有3例幽门螺杆菌感染。我们还研究了口腔MALT淋巴瘤的免疫组织化学。我们评估了H.唾液管中的幽门然而，需要进一步的调查，以了解致病机制。少