Molecular biological relationships between oral cavity and upper gastrointestinal tract in Helicobacter pylori infection

为了分析口腔内是否存在幽门螺杆菌（Helicobacter pylori， H. pylori），我们收集口腔疾病患者的牙菌斑和唾液，采用PCR技术检测幽门螺杆菌的存在。结果表明，幽门螺杆菌在口腔内的存在与年龄成正比，特别是在40岁以上的人群中，有50%的人检出幽门螺杆菌。接下来，为了了解口腔幽门螺杆菌对胃再感染的影响，以及口腔幽门螺杆菌患病率与胃根除成功的相关性，我们对感染患者在化疗时的牙菌斑控制情况进行了测试。结果，在根除治疗前后，4例患者中有3例口腔内检出幽门螺杆菌，而在牙菌斑控制后，口腔内未检出幽门螺杆菌。在胃中，经过根除治疗和牙菌斑控制后，所有患者在3、6和12个月的CLO试验均为阴性。我们的研究结果进一步表明，口腔幽门螺旋杆菌影响根除治疗的结果，并与胃感染的复发有关。我们建议在根除治疗前用PCR检查口腔幽门螺杆菌，如果阳性，应进行牙菌斑控制治疗。第三，我们研究了唾液在诱导和维持口服幽门螺杆菌疫苗小鼠免疫中的作用。因此，在口服免疫前进行涎腺切除术的小鼠口服疫苗接种的效果与在免疫完成后1个月进行涎腺切除术的小鼠相同，并且在免疫后6个月进行涎腺切除术的免疫效果明显降低。我们发现，唾液对幽门螺杆菌感染的保护和免疫的诱导和维持是必要的。为了研究口腔幽门螺杆菌患病率与口腔癌之间的关系，我们检测了免疫组织化学。本研究纳入了1994年至1998年间转介至京都大学医院的40例口腔肿瘤患者。结果，我们在40例患者中评估了3例幽门螺杆菌的存在。我们还检查了发生在口腔的MALT淋巴瘤的免疫组织化学。我们评估了唾液管中幽门螺杆菌的存在。但其致病机制尚需进一步研究。少

To analyze the existence of Helicobacter pylori (H. pylori) in the oral cavity, we collected the plaque and saliva in the patients with oral disease, and tested the existence of H. pylori, using PCR technique. As a result, H. pylori existed in the oral cavity in proportion to ages, especially which was detected in 50% among over 40 ages.Next, to know the influence of oral H. pylori on the gastric reinfection and the correlation between the prevalence of H. pylori in the oral cavity and the gastric eradication success, we tested the dental plaque control in infected patients, when they were treated with chemotherapy. As a result, three of four patients are detected with H. pylori in the oral cavity before and after eradication therapy, but H. pylori were not detected in the oral cavity after dental plaque control. In the stomach, after the treatment of eradication therapy followed by dental plaque control, all the patients were negative for CLO test in 3, 6 and 12 months. Our result sug … More gests that H. pylori in the oral cavity affect the outcome of eradication therapy and was associated with a recurrence of gastric infection. We recommend that oral H. pylori should be examined by PCR before eradication therapy, and if positive, should be treated with dental plaque control.Thirdly, we studied the role of saliva in the induction and maintenance of the immunity in mice conferred by oral vaccination against H. pylori. As a result, oral vaccination in the mice who underwent a sialoadenctomy before oral immunization was no less effective in the mice who underwent a sialoadenectomy after the completion of immunization at 1 month, and the efficacy of immunization was significantly reduced by sialoadenectomy at 6 months after immunization. We found that saliva is necessary about the protection from the H. pylori infection and immune induction and maintenance.To examine the correlation between the prevalence of H. pylori in the oral cavity and oral cancer, we examined immunohistochemistry. 40 patients who were referred to Kyoto University Hospital between 1994 and 1998 with oral tumor were enrolled in this study. As a result, we assessed the existence of H. pylori in 3 of 40 patients. We also examined immunohisochemistry in MALT lymphoma arising in the oral cavity. We assessed the existence of H. pylori in the salivary duct. However, further investigation is required to know about the pathogenic mechanism. Less