Characterization and genetic analysis of a newly developed grandmal seizure-prone rat NER

新开发的癫痫易发性大鼠 NER 的特征和遗传分析

基本信息

  • 批准号:
    08458275
  • 负责人:
  • 金额:
    $ 4.16万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    1996
  • 资助国家:
    日本
  • 起止时间:
    1996 至 1997
  • 项目状态:
    已结题

项目摘要

We characterized and evaluated as an animal model of epilepsy NER,a new epileptic rat strain, which was developed by inbreeding rats with spontaneous tonic-clonic seizures in a stock of Crj : Wistar. After the F9 generation, 94%-98% of NER exhibited spontaneous tonic-clonic convulsions, beginning with neck and forelimb clonus, wild jumping/running, opisthotonic posturing, and evolving to tonic, then clonic convulsion, followed by postictal flaccidity. Most seizure onsets occurred between 2-4 months of age, and the incidence was 0.45ア0.21 seizures in 12h. Ictal cortical and hippocampal EEGs were characterized by high-voltage spikes followed by diffuse spike-and wave or polyspike-and-wave complexes. NER revealed seizure susceptibility to pentylenetetrazol, tossing, and transcorneal electroshock. Mating experiments revealed that 0% (0/46) of the animals in F1,25.5% (13/51) in F2, and 63.6% (56/88) in backcross progenies exhibited spontaneous convulsions without sex difference. For all epileptic traits, no pathologic changes were demonstrated in the CNS.In order to confirm the mode of inheritance and locate the causative gene for epilepsy on the rat genome, we developed backcross progeny of NER with a seizure-resistant strain F344/DuCrj rats. The seizure propensity was specified by giving tossing stimuli. Linkage anaysis was made with whole genome scanning, and a significant association was found at D3M2Mit382 on chromosome 3. The spontaneous convulsions are comparable to generalized tonic-clonic seizures in human, and NER can serve as a new genetic model in epilepsy research.
我们的特点和评价作为一种动物模型的癫痫NER,一种新的癫痫大鼠品系,这是由近亲繁殖的大鼠自发强直-阵挛发作的股票Crj:Wistar。在F9代之后,94%-98%的NER表现出自发性强直-阵挛性惊厥,从颈部和前肢阵挛开始,疯狂跳跃/奔跑,强直性反张姿势,然后发展为强直性,然后是阵挛性惊厥,随后是发作后弛缓。癫痫发作多发生在2-4月龄,12 h内发作次数为0.45 ~ 0.21次。发作期皮层和海马脑电图的特点是高电压尖峰,其次是弥漫性棘波或多棘波复合体。NER显示癫痫发作的敏感性戊四氮,折腾,和transcorneal电击。交配试验表明,F1、F2和回交后代的惊厥发生率分别为0%(0/46)、25.5%(13/51)和63.6%(56/88),无性别差异。为了确认癫痫的遗传方式并在大鼠基因组中定位癫痫的致病基因,我们开发了NER与抗癫痫品系F344/DuCrj大鼠的回交后代。癫痫发作的倾向性是通过给予投掷刺激来确定的。利用全基因组扫描进行连锁分析,发现3号染色体上的D3 M2Mit 382位点与该位点有显著关联。自发性惊厥与人类全身性强直阵挛性发作相当,NER可以作为癫痫研究的一种新的遗传模型。

项目成果

期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kondo,Y.et.al.: "Genetic profile of three inbred strains derived from wild rats (Rattuw norbegicus) trapped in Japan." Exp.Anim.45. 405-409 (1996)
Kondo,Y.et.al.:“源自日本捕获的野生大鼠(Rattuwnorbegicus)的三种近交品系的遗传特征。”
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    0
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  • 通讯作者:
Momiyama,T.et.al.: "Long-term antiepileptic effcts of chronic intake of CNK-602A, a thyrotriopon-releasing hormone analogue,on spontaneously epileptic rats." Epilepsia. 37. 328-331 (1996)
Momiyama,T.et.al.:“长期摄入 CNK-602A(一种甲状腺激素释放激素类似物)对自发性癫痫大鼠的长期抗癫痫作用。”
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    0
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Hanaya, R.et al.: "Antiepileptic effects of 20-hydroxyecdysone on convulsive seizures in spontanneously epileptic rats." Jpn.J.Pharmacol.74. 331-335 (1997)
Hanaya, R.等人:“20-羟基蜕皮酮对自发性癫痫大鼠惊厥性癫痫发作的抗癫痫作用。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Iida, K.et al.: "Induction of convulsive seizures by acoustic priming in a new genetically defined model of epilepsy (Noda epileptic rat : NER)" Epilepsy Res.(in press). (1998)
Iida, K.等人:“在新的遗传定义的癫痫模型中通过声学启动诱导惊厥性癫痫发作(野田癫痫大鼠:NER)”癫痫研究(正在出版)。
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  • 影响因子:
    0
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  • 通讯作者:
Hanaya,R.et al.: "Suppression by topiramate of epileptiform burst discharges in hippocampal CA3 neurons of spontaneously epileptic rat (SER) in vivo." Brain Research. (in press). (1998)
Hanaya, R. 等人:“托吡酯对体内自发性癫痫大鼠 (SER) 海马 CA3 神经元的癫痫样爆发放电的抑制。”
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    0
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SERIKAWA Tadao其他文献

SERIKAWA Tadao的其他文献

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{{ truncateString('SERIKAWA Tadao', 18)}}的其他基金

Elucidation of the genetic factors in rat epileptic seizures
大鼠癫痫发作的遗传因素的阐明
  • 批准号:
    20240042
  • 财政年份:
    2008
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
CONSTRUCTION OF FINE COMPARTIVE GENETIC MAPS FOR THE RAT AND IDENTIFICATION OF CAUSATIVE GENES IN NEUROLOGICAL MUTANT RATS
大鼠精细比较遗传图谱的构建及神经突变大鼠致病基因的鉴定
  • 批准号:
    12308044
  • 财政年份:
    2000
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of genome analytical systems for identification of causative genes in rat models for human diseases
开发基因组分析系统,用于鉴定人类疾病大鼠模型中的致病基因
  • 批准号:
    09558105
  • 财政年份:
    1997
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of SSLP markers in rat models for diabetes and hypertension.
糖尿病和高血压大鼠模型中 SSLP 标记物的开发。
  • 批准号:
    07557352
  • 财政年份:
    1995
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
AMOLECULAR BIOLOGICAL STUDY FOR THE SPONTANEOUSLY EPILEPTIC RATS
自发性癫痫大鼠的分子生物学研究
  • 批准号:
    03454529
  • 财政年份:
    1991
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
A survey for Pneumocystis carinii in laboratory animal facilities
实验动物设施中卡氏肺孢子虫的调查
  • 批准号:
    63580038
  • 财政年份:
    1988
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
新しいてんかんモデルラットの開発
新型癫痫模型大鼠的研制
  • 批准号:
    61580037
  • 财政年份:
    1986
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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光遗传学迷走神经刺激装置在创伤后应激障碍动物模型中的研究
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