Development of SSLP markers in rat models for diabetes and hypertension.

大鼠已被广泛用作许多人类疾病的实验动物模型。它们在包括高血压和糖尿病在内的多因素疾病的研究中特别有用。为了确定这些类型的复杂疾病的发病机制所涉及的遗传位点，有必要建立详细的遗传图谱。因此，我们在本研究中建立了大鼠SSLP标志物。此外，我们在糖尿病或高血压大鼠模型中绘制了疾病相关基因座。大鼠SSLP标记我们开发了20个多态DNA标记（13个匿名DNA片段和7个大鼠基因）。此外，我们评估了来自Research Genetics提供的PCR引物，并在大鼠遗传图谱中定位了56个新的位点。利用小鼠SSLP引物，我们在大鼠中绘制了20个SSLP标记。大鼠糖尿病模型的遗传分析对BB/OK大鼠（1型糖尿病模型）的遗传分析表明，糖尿病易感性的第三个基因位于Olf位点附近（chr18）。在1型糖尿病新模型KDP大鼠中，我们在大鼠11号染色体上定位了一个主要的糖尿病易感基因（Iddm/kdp1）。在小鼠的16号染色体和人类的3.3号染色体上发现了该区域的保守性。大鼠高血压模型的遗传分析SER基因分析表明，Cryg基因座（chr9）与高血压密切相关。ppkata位点（chr8）与SER和DIS/Eis （Dahl大鼠）血压降低共分离。此外，在SHR中，Gja1位点（Chr 18）与体重共聚集。

The rat has been widely used as an experimental animal model for many human diseases. They are particularly useful in the study of multifactorial diseases including hypertension and diabetes. To identify genetic loci involved in the pathogenesis of these types of complex diseases, it is necessary to develop detailed genetic map. Therefore, we developed rat SSLP markers in this study. In addition, we mapped disease-related loci in rat models for diabetes or hypertension.1.Rat SSLP markersWe developed 20 polymorphic DNA markers (13 anonymous DNA fragments and 7 rat genes). In addition, we evaluated the PCR primer sets which were supplied from Research Genetics, and mapped 56 new loci in the rat genetic map. Using mouse SSLP primers, we mapped 20SSLP markers in the rat.2.Genetic analysis of rat models for diabetesGenetic analysis of BB/OK rat (a model for type 1 diabetes) suggested that the third gene for susceptibility of diabetes is located near Olf locus (Chr.18). In the KDP rat, a new model for type 1 diabetes, we mapped a major diabetes-susceptible gene (Iddm/kdp1) on the rat chromosome 11. The mapped region was found to be conserved on mouse choromosome 16 and human choromosome 3.3.Genetic analysis of rat models for hypertensionGenetic analysis of SER indicated that Cryg locus (Chr.9) were cosegragated with high blood pressure. Pkata locus (Chr.8) was cosegregated with decreased blood pressure in SER and DIS/Eis (Dahl rat). In addition, Gja1 locus(Chr 18) cosegeregated with body weight in SHR.