权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Basic studies on production of disease resistant plants by molecular control of phosphate metabolism

磷酸盐代谢分子控制生产抗病植物的基础研究

基本信息

批准号：
08556008
负责人：
EHARA Yoshio
金额：
$ 11.97万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1998
项目状态：
已结题

项目摘要

2,5-oligoadenylate synthease (2,5-AS) which induced in virus-infected animal cells forms 25-oligoadenytic acid [pppA(2p5A)n] from ATP.Transgenic plants (tobacco) introduced 2,5-AS gene showed resistance not only on viruses (cucumber mosaic virus and tobacco mosaic virus) but also on fungal diseases such as damping off which is cased by Rhizoctonia solani or powdery mildew disease which is caused by Erysiphe cichoracearum. The disease resistance was more promoted by treatment with phosphate salt (K- phosphate). The cell wall of the host plants treated with phosphate was thicker than that of untreated one. Thus, it was suggested that increase of resistance by treatment of phosphateis caused by inhibition of invasion by the fungi. In the transgenic plants with 2,5-AS gene, ribonuclease (RNase) activity increased more than in non-transgenic plants. The enzyme activity increased more by phosphate treatment, and it seemed also to act the inhibition of infection. The transgenic plants introduced cyclic AMP-non-depended protein kinase which produced in animal cells by interferon, showed resistance for diseases such as damping off and powdery mildew diseases. In these transgenic plants, ATP contents increased significantly. As mentioned above, it seemed that there are close relations between disease resistance and phosphate metabolism of plants. However, the relation between a bacterial disease and phosphate metabolism was not dear, so far as on fire blight (Pseudomonas syringae pv. tabaci) disease. From the present experimental results, it was confirmed that phosphate metabolism related closely to the plant resistance against virus and fungal diseases.

2,5-寡腺苷酸合成酶（2,5-AS）在病毒感染的动物细胞中诱导形成25-寡腺苷酸[pppA(2p5A)n]。引入2,5-AS基因的转基因植物（烟草）不仅对病毒（黄瓜花叶病毒和烟草花叶病毒）表现出抗性，而且对真菌病害（如病毒）也表现出抗性。如由立枯丝核菌引起的立枯病或由白粉病引起的白粉病。磷酸盐(K-磷酸盐)处理更能促进抗病性。用磷酸盐处理的宿主植物的细胞壁比未处理的宿主植物的细胞壁厚。因此，表明磷酸盐处理的抗性增加是由于抑制真菌的侵袭而引起的。在带有2,5-AS基因的转基因植物中，核糖核酸酶(RNase)活性比非转基因植物增加更多。磷酸盐处理后酶活性进一步增加，似乎也具有抑制感染的作用。转基因植物引入了由干扰素在动物细胞中产生的环AMP非依赖性蛋白激酶，表现出对立枯病和白粉病等疾病的抵抗力。在这些转基因植物中，ATP 含量显着增加。如上所述，植物的抗病性与磷代谢之间似乎存在着密切的关系。然而，就火疫病（Pseudomonas syringae pv. tabaci）病而言，细菌性疾病与磷酸盐代谢之间的关系并不明确。目前的实验结果证实，磷酸盐代谢与植物对病毒和真菌病害的抵抗力密切相关。