Molecular Evolution of Cell Compartment Specific Genes

细胞区室特异性基因的分子进化

• 批准号: 08640787
• 负责人: IWABE Naoyuki
• 金额: $ 1.15万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08640787/
• 关键词: molecular evolution; cell compartment; molecular phylogenetic tree; Giardia; rab; ADP-ribosylation factor; kinesin; ion pump; イオンポンプ

To know a possible relationship between organismal and molecular evolution, the divergence pattern of genes was examined based on a phylogenetic analysis of the GTP binding protein(GTPase)superfamily, the kinesin heavy chain family, and ion pump family by taking special notice of intracellular localization of these proteins. To obtain a rough estimate of times when these genes diverged by gene duplication, the cDNAs were cloned and sequenced from Giardia, a primitive protists lacking mitochondria as well as endoplasmic reticulum and Golgi apparatus, and we found that the major groups of members belonging to kinesin heavy chain, rab, and ADP-ribosylation factor families diverged by gene duplication before the separation of Giardia and other eukaryotes. A similar divergence pattern was also found in the calcium ion pump family. These results strongly suggest that most of gene duplications that gave rise to diverse genes with different intracellular localization have been completed in the very early evolution of eukaryotes. These results also suggest the secondary loss of endoplasmic reticulum and Golgi apparatus in Giardia lineage.

为了了解生物体和分子进化之间可能存在的关系，基于GTP结合蛋白（GTbinding protein，GTbinding protein）超家族、驱动蛋白重链家族和离子泵家族的系统发育分析，通过特别注意这些蛋白质的细胞内定位，研究了基因的分化模式。为了获得一个粗略的估计时间，当这些基因分歧的基因复制，cDNAs克隆和测序贾第虫，一个原始的原生生物缺乏线粒体以及内质网和高尔基体，我们发现，主要群体的成员属于驱动蛋白重链，rab和ADP-核糖基化因子家族分歧的基因复制之前，贾第虫和其他真核生物分离。在钙离子泵家族中也发现了类似的发散模式。这些结果有力地表明，大多数导致不同基因具有不同细胞内定位的基因复制已经在真核生物的非常早期的进化中完成。这些结果也提示了贾第鞭毛虫谱系内质网和高尔基体的继发性缺失。

项目成果

Nikoh N.et al.: "An estimate of divergence time of Parazoa and Eumetazoa and that of Cephalochordata and Vertebrata by aldolase and triose phosphate isomerase clocks." J.Mol.Evol.45(1). 97-106. (1997)

Nikoh N.等人：“通过醛缩酶和磷酸丙糖异构酶时钟估计副动物门和真动物门以及头索动物门和脊椎动物门的分歧时间。”

Suga, H.et al.: "Intermittent sivergence of the protein tyrosine kinase family during animal evolution." FEBS Lett.412. 540-546 (1997)

Suga, H.et al.：“动物进化过程中蛋白质酪氨酸激酶家族的间歇性收敛。”

Iwabe, N.et al.: "Evolution of Gene Families and Relationship with Organismal Evolution : Rapid Divergence of Tissue-Specific Genes in the Early Evolution of Chordates." Molecular Biology and Evolution. 13(3). 483-493 (1996)

Iwabe, N.等人：“基因家族的进化及其与生物体进化的关系：脊索动物早期进化中组织特异性基因的快速分化。”

Suga, H.et al.: "Intermittent divergence of the protein tyrosine kinase family during animal evolution" FEBS Lett.412(3). 540-546 (1997)

Suga, H.et al.：“动物进化过程中蛋白酪氨酸激酶家族的间歇性分歧”FEBS Lett.412(3)。

Iwabe, N., et al.: "Evolution of gene families and relationship with organismal evolution : rapid divergence of tissue specific genes in the early evolution of chordates." Mol.Biol.Evol.13(3). 483-493 (1996)

Iwabe, N. 等人：“基因家族的进化及其与有机体进化的关系：脊索动物早期进化中组织特异性基因的快速分化。”